ABE446

Anti-Monomethyl Histone H1 (Lys26) Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Histone H1.3, Histone H1c, Histone H1s-2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 기술: dot blot: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: NP_005311
• UniProt 수납 번호: P16402
• 배송 상태: wet ice
• 타겟 번역 후 변형: monomethylation (Lys26)
• 유전자 정보: human ... HIST1H1D(3007)

설명

일반 설명

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes, and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.

특이성

This antibody recognizes the N-terminus of Monomethyl Histone H1 (Lys26) methylated at Lys26.

면역원

KLH-conjugated linear peptide corresponding to the N-terminus of human Monomethyl Histone H1 (Lys26).

애플리케이션

Detect Histone H1 using this rabbit polyclonal antibody, Anti-Monomethyl Histone H1 (Lys26) Antibody validated for use in western blotting, Dot Blot & IP.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Monomethyl Histone H1 (Lys26) in 500 µg of HeLa acid extract.
Dot Blot Specificity Analysis: This antibody detected Monomethyl Histone H1 (Lys26) in in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and in an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

품질

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Monomethyl Histone H1 (Lys26) in 10 µg of HeLa acid extract.

표적 설명

~32 kDa observed

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable