추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
mouse, human
기술
ChIP: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
mutation (Lys27Met)
유전자 정보
human ... H3F3B(3021)
일반 설명
Histone H3.3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3.3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.
특이성
This antibody recognizes Histone H3.3 with K27M mutation.
면역원
Epitope: Histsone H3 sequence surrounding K27M mutation
KLH-conjugated linear peptide corresponding to sequence near the N-terminus of human Histone H3.3 with K27M mutation.
애플리케이션
- Peptide Inhibition Assay (PIA): Target band detection inlysate from HEK-293 cells transfected with Histone H3.3 K27M mutant wasprevented by pre-blocking of a representative lot with the immunogen HistoneH3.3 K27M mutant peptide, but not the corresponding unmodified Histone H3.3 peptide.
- Immunohistochemistry (IHC): A representative lot of this antibodydetected Histone 3.3 K27M in pediatric glioblastoma tissue sections. (Venneti,S., et al. (2014). Acta Neuropathol 128(5); 743-753).
Anti-Histone H3.3 Antibody, K27M mutant, is validated for use in western blotting (WB) & Chromatin immunoprecipitation (ChIP).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
품질
Evaluated by Western Blotting in HEK-293 cellstransfected with Histone H3.3 K27M.
Western Blotting Analysis (WB): A 1:2,000 dilution of a representative lot of thisantibody detected Histone H3.3 K27M in HEK-293 cells transfected with HistoneH3.3 K27M mutant.
Western Blotting Analysis (WB): A 1:2,000 dilution of a representative lot of thisantibody detected Histone H3.3 K27M in HEK-293 cells transfected with HistoneH3.3 K27M mutant.
표적 설명
~17 kDa observed
물리적 형태
Immunogen Affinity Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
분석 메모
Control
Lysates from MEF transfectants expressing K27M (positive) or wildtype (negative) FLAG-HA-tagged histone H3.3.
Lysates from MEF transfectants expressing K27M (positive) or wildtype (negative) FLAG-HA-tagged histone H3.3.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Melanie Pages et al.
Acta neuropathologica communications, 3, 85-85 (2015-12-17)
Papillary Glioneuronal Tumor (PGNT) is a grade I tumor which was classified as a separate entity in the World Health Organization Classification of the Central Nervous System 2007 in the group of mixed glioneuronal tumors. This tumor is rare and
Tina Y Huang et al.
Acta neuropathologica communications, 5(1), 28-28 (2017-04-19)
Diffuse midline gliomas (including diffuse intrinsic pontine glioma, DIPG) are highly morbid glial neoplasms of the thalamus or brainstem that typically arise in young children and are not surgically resectable. These tumors are characterized by a high rate of histone
Anthony P Y Liu et al.
Acta neuropathologica communications, 6(1), 101-101 (2018-09-27)
Tectal glioma (TG) is a rare low-grade tumor occurring predominantly in the pediatric population. There has been no detailed analysis of molecular alterations in TG. Risk factors associated with inferior outcome and long-term sequelae of TG have not been well-documented.
Specific detection of methionine 27 mutation in histone 3 variants (H3K27M) in fixed tissue from high-grade astrocytomas.
Bechet, D; Gielen, GG; Korshunov, A; Pfister, SM; Rousso, C; Faury, D; Fiset et al.
Acta neuropathologica null
Karisa C Schreck et al.
Journal of neuro-oncology, 143(1), 87-93 (2019-03-14)
H3 K27 mutations, most commonly in H3F3A, are common in diffuse midline glioma. The exact frequency of these mutations in adults with gliomas in the midline location is unknown. This study was conducted to define the incidence of H3 K27M
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