ABE2984

Anti-Phospho-RXRα/RXRA (Ser56)

• 동의어(들): Nuclear receptor subfamily 2 group B member 1, Retinoic acid receptor RXR-alpha, Retinoid X receptor alpha
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 분자량: calculated mol wt 50.81 kDa; observed mol wt ~75-80 kDa
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): bovine, rat, mouse
• 포장: antibody small pack of 100
• 기술: dot blot: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG
• 에피토프 서열: N-terminal
• 단백질 ID 수납 번호: NP_001278850.1
• UniProt 수납 번호: P19793
• 저장 온도: 2-8°C
• 유전자 정보: human ... RXRA(6256)

설명

특이성

This rabbit polyclonal antibody detects RXR phosphorylated on serine 56.

면역원

KLH-conjugated linear peptide corresponding to 12 amino acids from the surrounding phosphoserine 56 from the N-terminal region of human Retinoic acid receptor (RXR ).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in lysate from HeLa cells in mitotic phase.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RXR phosphorylated on serine 56 in lysate from HeLa cells in mitotic phase.

Tested Applications

Immunofluorescence Analysis: A representative lot detected RXR in Immunofluorescence application (Xie, G., et al. (2021). STAR Protocols. 2, :1-15).

Western Blotting Analysis: A representative lot detected RXR in Western Blotting application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Immunoprecipitation Analysis: A representative lot immunoprecipitated RXR in Immunoprecipitation application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Immunocytochemistry Analysis: A representative lot detected RXR in Immunocytochemistry application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Flow Cytometry Analysis: A representative lot detected RXR in Flow Cytometry application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Dot Blot: A representative lot detected RXR in Dot Blot application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Flow Cytometry Analysis: 1.0 µg of this antibody detected RXR in HeLa cells overnight serum-starved and treated with Nocodazole 100 ng/ml for 8 hours .

Immunohistochemistry Applications: A representative lot detected RXR in Immunohistochemistry application (Xie, G., et al. (2020). Development Cell. 55, : 707-722).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Retinoic acid receptor RXR-alpha (UniProt: P19793; also known as Nuclear receptor subfamily 2 group B member 1, Retinoid X receptor alpha, RXR ) is encoded by the RXRA (also known as NR2B1) gene (Gene ID: 6256) in human. Retinoid X receptor alpha (RXR ), a nuclear receptor of transcription factor, controls various physiological and pathological pathways including cellular growth, proliferation, differentiation, and apoptosis. RXR-alpha is composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain, and a C-terminal ligand-binding domain (AF2 domain). RXR-alpha protein is highly expressed in liver and its expression has also been reported in lung fibroblasts, monocytes, kidney, and brain. RXR is phosphorylated on serine and threonine residues mainly at its N-terminal A/B domain by cyclin-dependent kinase 1 (Cdk1) at the onset of mitosis, triggering its translocation to the centrosome, where phosphorylated-RXR (p-RXR ) interacts with polo-like kinase 1 (PLK1) through its N-terminal A/B domain by a unique mechanism. The interaction promotes PLK1 activation, centrosome maturation, and mitotic progression. Studies show that Cdk1 phosphorylation of Ser56 and Ser70 mediates the interaction between p-RXR and PLK1 during mitosis. Importantly, Ser56 and Ser70 and their surrounding amino acids are highly conserved across different species, suggesting their evolutionarily conserved role in regulating RXR activity. An RXR ligand XS060, which specifically inhibits p-RXR /PLK1 interaction but not RXR heterodimerization, promotes mitotic arrest and catastrophe in a tumor-specific manner. Levels of p-RXR are abnormally elevated in cancer cell lines, during carcinogenesis in animals, and in clinical tumor tissues. (Ref.: Xie, G., et al. (2020). Development Cell. 55(6); 707-722; Xie, G., et al. (2021). STAR Protocols. 2(3); 1-15; Zeng, Z., et al. (2015). Cancer Res. 75(10); 2049-2060.)

물리적 형태

Purified rabbit polyclonal antibody in PBS without azide.

재구성

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable