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Merck
모든 사진(3)

Key Documents

ABE2901

Sigma-Aldrich

Anti-Dcp2

serum, from rabbit

동의어(들):

m7GpppN-mRNA hydrolase, EC:3.6.1.62, Nucleoside diphosphate-linked moiety X motif 20, Nudix motif 20, mRNA-decapping enzyme 2, hDpc

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

항체 형태

serum

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

mouse, human

포장

antibody small pack of 25 μL

기술

western blot: suitable

동형

IgG

NCBI 수납 번호

UniProt 수납 번호

타겟 번역 후 변형

unmodified

유전자 정보

human ... DCP2(167227)

일반 설명

m7GpppN-mRNA hydrolase (UniProt: Q8IU60; also known as EC:3.6.1.62, Nucleoside diphosphate-linked moiety X motif 20, Nudix motif 20, mRNA-decapping enzyme 2, hDpc) is encoded by the DCP2 (also known as NUDT20) gene (Gene ID: 167227) in human. Dcp2 is a manganese-dependent decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. It removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5′-phosphorylated mRNA fragment and 7m-GDP. Dcp2 contains a functional Nudix (nucleotide diphosphate linked to an X moiety) motif (aa 95-226) that is essential for its decapping activity. Two isoforms of Dcp2 have been described that are produced by alternative splicing. Dcp2 is reported to play a role in replication-dependent histone mRNA degradation. It has higher activity towards mRNAs that lack a poly(A) tail and does not display any activity towards a cap structure lacking an RNA moiety. Dcp2 is predominantly cytoplasmic, however, a small amount may also be found in the nucleus. Higher expression of Dcp2 has been reported in subjects with celiac disease with higher levels in the nuclear fraction. (Ref.: Wang, Z., et al. (2002). Proc. Natl. Acad. Sci. USA 99(20); 12663-12668; Castellanos-Rubio, A., et al. (2016). Science 352(6281); 91-95).

특이성

This rabbit polyclonal antibody detects m7GpppN-mRNA hydrolase (Dcp2) in human and murine cells.

면역원

Epitope: unknown
His-tagged full length recombinant human m7GpppN-mRNA hydrolase (Dcp2).

애플리케이션

Anti-Dcp2, Cat. No. ABE2901, is a highly specific rabbit polyclonal antibody that targets m7GpppN-mRNA hydrolase / NUDT20 (Dcp2) and has been tested for use in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected Dcp2 in 293T KO, K562, 293T and MEF DCP 2 lysates (Courtesy of Dr. Mike Kiledjian at Rutgers University).

Western Blotting Analysis: A representative lot detected Dcp2 in Western Blotting applications (Castellanos-Rubio, A., et. al. (2016). Science. 352(6281):91-5; Li, Y., et. al. (2012). Mol Cell Biol. 32(6):1164-72; Jiao, X., et. al. (2006). Mol Cell. 24(5):713-22; Li, Y., et. al. (2008). Mol Cell Biol. 28(3):939-48; Song, M.G., et. al. (2010). Mol Cell. 40(3):423-32; Li, Y., et. al. (2012). Mol Cell Biol. 32(6):1164-72).

품질

Evaluated by Western Blotting in 293T control KO cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Dcp2 (NUDT20) in lysate from 293T control knockout cells, but did not react with Dcp2 knockout 293T cell lysate.

표적 설명

~51 kDa observed; 48.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Rabbit polyclonal antibody in serum with 0.05% sodium azide.
Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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