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Merck
모든 사진(3)

주요 문서

ABE2868M

Sigma-Aldrich

Anti-SOX9 Antibody

rabbit polyclonal

동의어(들):

Transcription factor SOX-9

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

제품명

Anti-Sox9, from rabbit

생물학적 소스

rabbit

Quality Level

항체 형태

unpurified

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

rat, mouse, human

포장

antibody small pack of 25 μL

기술

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

동형

IgG

NCBI 수납 번호

UniProt 수납 번호

타겟 번역 후 변형

unmodified

유전자 정보

human ... SOX9(6662)

일반 설명

Transcription factor SOX-9 (UniProt P48436; also known SOX-9) is encoded by the SOX9 gene (Gene ID 6662) in human. SOX family of genes are related to the mammalian sex determining gene SRY. These genes contain sequences encoding for the HMG-box domains that mediate sequence-specific DNA binding. SOX genes encode transcriptional regulators involved in cell fate decision during development and the control of diverse developmental processes. The highly complex group of SOX genes cluster at about 40 genomic loci and at least 30 SOX genes have been identified. SOX-9 contains one HMG box DNA-binding domain and acts as a key regulator of developmental processes, including male sex determination, normal skeletal development, chondrogenesis, neurogenesis, and neural crest development. SOX-9 is acts downstream of sonic hedgehog signaling and is shown to be essential for the maintenance of multipotent neural stem cells in embryonic and adult central nervous system (CNS). It is not detected in neuroblasts of the adult subependymal zone (SEZ); however, it is expressed by transit amplifying cells and ependymal cells in the same region. SOX-9 is also shown to be essential for the ′switch′ from neurogenesis to gliogenesis in caudal regions of the developing CNS. SOX-9 up-regulation is reported in cancers of various origins, including breast, colon, prostate, and lung, where its expression correlates with malignancy and patient survival. (Ref.: Scott, CE et al. (2010). Nat. Neurosci. 13(10): 1181-89).

특이성

This rabbit ployclonal antibody detects SOX-9 in human, mouse, and rat cells. It targets an epitope with in 24 amino acids from the C-terminal end. This antibdy does not recognize Sox10.

면역원

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal end of human Sox-9.

애플리케이션

Anti-Sox9, Cat. No. ABE2868, is a highly specific rabbit polyclonal antibody that targets Transcription factor SOX-9 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A 1:500 dilution from a representative lot detected SOX-9 in 10 µg of HepG2 cell lysate.

Western Blotting Analysis: A representative lot detected SOX-9 in Western Blotting applications (Augereau, C., et. al. (2016). Hum Mol Genet. Epub ahead of print).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected SOX-9 in HEK293T cells transfected with Sox-9 (Courtesy of Dr. Patrick Jacquemin atUniversité catholique de Louvain).

Immunofluorescence Analysis: A representative lot detected SOX-9 in PFA-fixed, paraffin-embedded pancreatic sections from an adult mouse; ductus (Augereau, C., et. al. (2016). Hum Mol Genet. 25(22):5017-5026).

Immunohistochemistry Analysis: A representative lot detected SOX-9 in PFA-fixed, paraffin-embedded liver (left) and pancreatic sections (right) of E14.5 mouse embryo, PFA-fixed, paraffin-embedded intestine sections of E14.5 mouse embryo (Augereau, C., et. al. (2016). Hum Mol Genet. 25(22):5017-5026).

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SOX-9 in e14.5 mouse liver and e14.5 mouse pancreas tissues.

Immunocytochemistry Analysis: A representative lot detected SOX-9 in PFA-fixed 3T3 cells overexpressing Sox9 (Augereau, C., et. al. (2016). Hum Mol Genet. 25(22):5017-5026).

품질

Evaluated by Western Blotting in L6 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected SOX-9 in 10 µg of L6 cell lysate.

표적 설명

~65 kDa observed; 56.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Unpurified
Rabbit polyclonal antiserum with 0.02% sodium azide.
Unpurified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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