ABE281

Anti-EZH1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: polyclonal
• application: IHC; WB
• 종 반응성: human, mouse
• 기술: immunohistochemistry: suitable; western blot: suitable
• citations: 6

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, mouse
• 종 반응성(상동성에 의해 예측): rat (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• 기술: immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_001982
• UniProt 수납 번호: Q92800
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... EZH1(2145)

설명

일반 설명

Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH1 is the catalytic subunit of the PRC2/EED-EZH1 complex. This complex methylates Lys-27 of histone H3, which leads to transcriptional repression of the affected target gene. EZH1 is required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in maintaining the identity of embryonic stem cells.

특이성

Other homologies: Porcine (94% sequence homology).

This antibody potentially recognizes all isoforms of EZH1.

면역원

KLH-conjugated linear peptide corresponding to human EZH1.

애플리케이션

Detect the EZH1 protein using this Anti-EZH1 validated for use in IHC & WB.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected EZH1 in human lung and kidney tissues.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

품질

Evaluated by Western Blot in mouse embryonic stem cell lysate.

Western Blot Analysis: 0.2 µg/mL of this antibody detected EZH1 in 10 µg of mouse embryonic stem cell lysate.

표적 설명

~85 kDa observed. Uniprot states 5 isoforms for EZH1. This antibody potentially recognizes all isoforms. Isoform A is 85,271 Daltons. Isoform B is 86,025 Daltons. Isoform C is 80,699 Daltons. Isoform D is 76,952 Daltons. Isoform E is 68,961 Daltons. (Margueron, 2008).

결합

Replaces: 04-1047

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Mouse embryonic stem cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable