ABE278

Anti-MILI Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Piwi-like protein 2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: polyclonal
• application: ICC; IHC; IP; WB
• 종 반응성: rat, mouse
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: rat, mouse
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: NP_067283
• UniProt 수납 번호: Q8CDG1
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Piwil2(57746) ; rat ... Piwil2(306011)

설명

일반 설명

MILI, MIWI, and MIWI2 belong to the Piwi family of proteins. piRNAs and Piwi proteins are implicated in transposon control and are linked to transposon methylation in mammals. MILI, in particular, directly binds with piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. MILI plays an essential role in spermatogenesis, meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells.

면역원

KLH-conjugated linear peptide corresponding to mouse MILI.

애플리케이션

Anti-MILI Antibody is a Rabbit Polyclonal Antibody for detection of MILI also known as Piwi-like protein 2 & has been validated in WB, ICC, IHC & IP.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in male primordial germ cells. (Aravin, A.A., et al. (2008). Mol Cell. 31(6):785-799.)

Immunohistochemistry Fluoresence Analysis: A representative lot was used by an independent laboratory in embyronic testis cross sections. (Vagin, W., et al. (2009). Genes Dev. 23(15):1749-1762.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory. (Aravin, A.A., et al. (2007). Science. 316(5825):744-747.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

품질

Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected MILI in 10 µg of RAW264.7 cell lysate.

표적 설명

~109 kDa observed. An uncharacterized bands at ~30, 55, and 60 kDa may be observed in some lysates.

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
RAW264.7 cell lysate

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable