ABE1877M

Anti-PAXIP1

from rabbit

• 동의어(들): PAX-interacting protein 1, PAX transactivation activation domain-interacting protein, PAXIP
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, mouse
• 포장: antibody small pack of 25 μL
• 기술: ChIP: suitable (ChIP-seq); immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_061366
• UniProt 수납 번호: Q6NZQ4
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PAXIP1(22976) ; mouse ... Paxip1(55982)

설명

일반 설명

PAX-interacting protein 1 (UniProt: Q6NZQ4; also known as PAX transactivation activation domain-interacting protein, PAXIP1) is encoded by the Paxip1 (also known as Ptip) gene (Gene ID: 55982) in murine species. PAXIP1 is a nuclear matrix protein that is involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes such as the MLL2/MLL3 complex. In vitro it is shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks. It recruits Tp53bp1 to DNA damage foci and effective DNA damage response appears to require the association with Tp53bp1 phosphorylated by ATM. It also recruits PAGR1A (Pax-Interacting Protein 1-associated glutamate rich protein 1a) to DNA damage sites and PAGR1A:PAXIP1 complex is believed to be essential for cell survival. PAXIP1 expression is reported in all tissues and high expression is observed in embryonic kidney and brain. PAXIP1 contains 6 BRCT domains and BRCT domains 1 and 2 mediate its interaction with PAGR1A and interaction with MLL2/MLL3 is accomplished with BRCT domains 5 and 6. Mice with defective Paxip1 gene are developmentally retarded and usually face embryonic lethality by day 9.5 (E9.5).

특이성

This rabbit polyclonal antibody detects PAX-interacting protein 1 in human and murine cells. It targets an epitope within 199 amino acids from the N-terminal region.

면역원

Epitope: unknown

His-tagged recombinant fragment corresponding to 199 amino acids from the N-terminal region of murine PAX-interacting protein 1.

애플리케이션

Anti-PAXIP1, Cat. No. ABE1877, is a highly specific rabbit polyclonal antibody that targets PAX-interacting protein 1 and has been tested for use in ChIP-seq and Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

Chromatin Immunoprecipitation Analysis: A representative lot detected PAXIP1 in mouse embryonic fibroblasts (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39).

ChIP-seq Analysis: Analysis: A representative lot detected PAXIP1 in ChIP-seq applications (Callen, E., et. al. (2012). Immunity. 37(6):971-85).

Immunoprecipitation Analysis: A representative lot detected PAXIP1 in Immunoprecipitation applications (Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).

Immunoprecipitation Analysis: 1 µg from a representative lot detected PAXIP1 in nuclear extract of wild type and PTIP KO mouse embryonic fibroblasts (Courtesy of Dr. Ji-Eun Lee and Dr. Kai Ge at NIH).

Western Blotting Analysis: A representative lot detected PAXIP1 in Western Blotting applications (Cho, Y.W., et. al. (2009). Cell Metab. 10(1):27-39; Gong, Z., et. al. (2009). J Biol Chem. 284(11):7284-93).

Research Category
Epigenetics & Nuclear Function

품질

Evaluated by Western Blotting with with recombinant Pax-interacting protein 1.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant PAX-interacting protein 1.

표적 설명

~51 kDa observed (recombinant); 119.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1