ABE1331

Anti-Zinc finger protein Eos Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Zinc finger protein Eos, Ikaros family zinc finger protein 4
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): mouse (based on 100% sequence homology)
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_071910
• UniProt 수납 번호: Q9H2S9
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... IKZF4(64375)

설명

일반 설명

Zinc finger protein Eos, also known as Zinc finger protein Eos, or Ikaros family zinc finger protein 4, and encoded by the gene name IKZF4/ KIAA1782/ ZNFN1A4, is a member of the Ikaros C2H2-type zinc-finger protein family. Zinc finger protein Eos is a DNA-binding protein that binds to the 5′GGGAATRCC-3′ Ikaros-binding sequence. It is also a transcriptional repressor, which interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. Zinc finger protein Eos may be involved in the development of central and peripheral nervous systems. Zinc finger protein Eos interacts with other family members: IKZF1, IKZF2, IKZF3 AND IKZF5; as well as CTBP2, SPI1 and MITF. Zinc finger protein Eos is highly expressed in skeletal muscle, and with low levels of expression in heart, thymus, kidney, liver, and spleen. Zinc finger protein Eos is also expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K-562. High level of expression has been detected in THP-1 and M-07e cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively.

특이성

Immunogen sequence is 93% homologous to Bovine.

면역원

KLH-conjugated linear peptide corresponding to human Zinc finger protein Eos near the C-terminus.

애플리케이션

This Anti-Zinc finger protein Eos antibody is validated for use in WB, IC for the detection of Zinc finger protein Eos.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Zinc finger protein Eos in 10 µg of human thymus tissue lysate.
Immunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Zinc finger protein Eos in HepG2 cells.

품질

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Zinc finger protein Eos in 10 µg of HepG2 cell lysate.

표적 설명

~60 kDa observed. This antibody is predicted to detect both isoforms of Zinc finger protein Eos (~64 & ~59 kDa) based on immunogen sequence. Uncharacterized band(s) may appear in some lysates.

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable