추천 제품
생물학적 소스
rabbit
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
rat
포장
antibody small pack of 25 μL
기술
dot blot: suitable
immunofluorescence: suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
타겟 번역 후 변형
phosphorylation (pTyr1418)
유전자 정보
rat ... Dcc(25311)
일반 설명
Netrin receptor DCC (UniProt: Q63155; also known as Tumor suppressor protein DCC, Deleted in Colorectal Cancer) is encoded by the Dcc gene (Gene ID: 25311) in rat. Netrin receptor DCC is a single-pass type I membrane protein that serves as a receptor for netrin and is essential for axon guidance. In response to netrin-1 stimulation, DCC becomes a signaling platform to recruit proteins that promote axon outgrowth and guidance. It is detected in embryonic spinal cord, predominantly in axons of commissural neurons. Deficiencies in netrin-1 or DCC expression can result in the loss of cerebral and spinal commissures. Netrin receptor DCC also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand and has been implicated as a tumor suppressor gene. It is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off. It has four Ig-like C2-type domains and six fibronectin type-III domains. In response to netrin-1 stimulation DCC is phosphorylated on serine, threonine, and tyrosine residues. Its phosphorylation on Tyrosine 1418 by Src family kinases is shown to be essential for netrin-1 to mediate axon outgrowth and guidance. It has been reported that in response to netrin-1, p120RasGAP is recruited to DCC in growth cones and forms a multiprotein complex with focal adhesion kinase (FAK) and extracellular signal regulated kinase (ERK). Activities of both Ras and ERK as shown to be elevated in p120RasGAP deficient neurons. (Ref.: Antoine-Bertrand J., et al., (2016). J. Biol. Chem. 291(9):4589-602).
특이성
This rabbit polyclonal antibody detects Netrin receptor DCC phosphorylalted on Tyrosine 1418 in rat cells. It targets an epitope within 15 amino acids from the C-terminal region.
면역원
A linear peptide corresponding to 15 amino acids surrounding phospho-tyrosine 1418 from the C-terminal region of rat Netrin receptor DCC.
Epitope: C-terminus
애플리케이션
Anti-phospho DCC (Tyr1418), Cat. No. ABC1466, iis a rabit polyclonal antibody that detects Netrin receptor DCC and has been tested for use in Dot Blot, Immunofluorescence, and Western Blotting.
Dot Blot Analysis: A representative lot detected phospho DCC (Tyr1418) in Dot Blot applications (Antoine-Bertrand, J., et. al. (2016). J Biol Chem. 291(9):4589-602).
Immunofluorescence Analysis: A representative lot detected phospho DCC (Tyr1418) in Immunofluorescence applications (Plooster, M., et. al. (2017). Mol Biol Cell. 28(18):2374-2385).
Western Blotting Analysis: A representative lot detected phospho DCC (Tyr1418) in Western Blotting applications (Plooster, M., et. al. (2017). Mol Biol Cell. 28(18):2374-2385; Petrie, R.J., et. al. (2009). Biol Cell. 101(2):77-90).
Immunofluorescence Analysis: A representative lot detected phospho DCC (Tyr1418) in Immunofluorescence applications (Plooster, M., et. al. (2017). Mol Biol Cell. 28(18):2374-2385).
Western Blotting Analysis: A representative lot detected phospho DCC (Tyr1418) in Western Blotting applications (Plooster, M., et. al. (2017). Mol Biol Cell. 28(18):2374-2385; Petrie, R.J., et. al. (2009). Biol Cell. 101(2):77-90).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
품질
Evaluated by Western Blotting in HEK293 transfected with pRK5-DCC (DCCWT).
Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho DCC (Tyr1418) in HEK293 transfected wtih pRK5-DCC (DCCWT).
Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho DCC (Tyr1418) in HEK293 transfected wtih pRK5-DCC (DCCWT).
표적 설명
~190 kDa observed; 157.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
물리적 형태
Rabbit polyclonal antiserum with 0.05% sodium azide with 20% glycerol.
Unpurified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
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