ABC1428

Anti-RecQ1

from rabbit

• 동의어(들): ATP-dependent DNA helicase Q1, RecQ-like type 1, DNA-dependent ATPase Q1, RecQ protein-like 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human
• 기술: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_116559
• UniProt 수납 번호: P46063
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... RECQL(5965)

설명

일반 설명

ATP-dependent DNA helicase Q1 (UniProt: P46063; also known as EC:3.6.4.12, DNA helicase, RecQ-like type 1, RecQ1, DNA-dependent ATPase Q1, RecQ protein-like 1) is encoded by the RECQL (also known as RECQ1, RECQL1) gene (Gene ID: 5965) in human. RecQ helicases are a ubiquitous family of DNA unwinding enzymes involved in the maintenance of chromosome stability. They participate in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Five members of the RecQ family have been reported in human cells: BLM, RECQ1, RECQ4, RECQ5, and WRN. RecQ1 displays high expression in heart, lung, skeletal muscle and kidney. It is also highly expressed in tumor cells and although brain exhibits low level of EecQ1, its expression is dramatically increased in human glioblastoma. Depletion of RecQ1 affects proliferation of glioblastoma cells and causes an increase in the level of DNA damage. RecQ1 depleted tumor cells are shown to be more sensitive to anti-tumor agents, such as hydroxyurea and temozolomide.

특이성

This rabbit polyclonal antibody detects ATP-dependent DNA helicase Q1 (RecQ1) in human cells. It targets an epitope within 16 amino acids from the C-terminal end.

면역원

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human ATP-dependent DNA helicase Q1 (RecQ1).

애플리케이션

Anti-RecQ1, Cat. No. ABC1428, is a rabbit polyclonal antibody that detects ATP-dependent DNA helicase Q1 and has been tested for use in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected RecQ1 in T98G cells after treatment with anti-RECQ1 siRNA or control siRNAs (Mendoza-Maldonado, R., et. al. (2011). Mol Cancer. 10:83).

Western Blotting Analysis: A representative lot detected RecQ1 in Western Blotting applications (Thangavel, S., et. al. (2015). J Cell Biol. 208(5):545-62).

Immunohistochemistry Analysis: A representative lot detected RecQ1 in tissues are perilesional sections from multiform glioblastoma, colon carcinoma,lung cancer and thyroid cancer (Mendoza-Maldonado, R., et. al. (2011). Mol Cancer. 10:83).

Research Category
Apoptosis & Cancer

품질

Evaluated by Western Blotting in IMR90 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RecQ1 in 10 µg of IMR90 human fetal lung cell lysate.

표적 설명

~75 kDa observed; 73.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing Tris-citrate-phosphate buffer (pH 7.8) with 0.09% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable