생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
rat
제조업체/상표
Chemicon®
기술
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
rat ... Basp1(64160)
특이성
Recognizes NAP-22 [Neuronal acidic protein 22, CAP-23, BASP1].
면역원
Epitope: Complete sequence
Recombinant full length rat NAP-22.
애플리케이션
Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 55 kDa NAP-22 protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 10%. Overnight incubation with the antibody is recommended.
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Neuroregenerative Medicine
Developmental Neuroscience
Neuroregenerative Medicine
This Anti-NAP-22 Antibody, complete sequence is validated for use in WB for the detection of NAP-22.
물리적 형태
Format: Purified
Semi-purified immunoglobulin. Liquid in TBS containing 0.05% sodium azide.
저장 및 안정성
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
분석 메모
Control
POSITIVE CONTROL: Neonatal rat brain extract. NAP-22 is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze NAP-22 content by immunoblot.
POSITIVE CONTROL: Neonatal rat brain extract. NAP-22 is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze NAP-22 content by immunoblot.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
T Laux et al.
The Journal of cell biology, 149(7), 1455-1472 (2000-06-28)
The dynamic properties of the cell cortex and its actin cytoskeleton determine important aspects of cell behavior and are a major target of cell regulation. GAP43, myristoylated alanine-rich C kinase substrate (MARCKS), and CAP23 (GMC) are locally abundant, plasmalemma-associated PKC
D Frey et al.
The Journal of cell biology, 149(7), 1443-1454 (2000-06-28)
CAP23 is a major cortical cytoskeleton-associated and calmodulin binding protein that is widely and abundantly expressed during development, maintained in selected brain structures in the adult, and reinduced during nerve regeneration. Overexpression of CAP23 in adult neurons of transgenic mice
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