AB5731

Anti-Nanog Antibody, NT

Chemicon^®, from rabbit

• 동의어(들): Homeobox transcription factor Nanog, Nanog homeobox
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, rat, mouse
• 포장: antibody small pack of 25 μg
• 제조업체/상표: Chemicon^®
• 기술: western blot: suitable
• 입력: sample type: mouse embryonic stem cell(s); sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• 동형: IgG
• NCBI 수납 번호: NM_024865.2
• UniProt 수납 번호: Q9H9S0
• 배송 상태: ambient
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... NANOG(79923) ; mouse ... Nanog(71950)

설명

일반 설명

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency, i.e., these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
NANOG may be useful in the immunohistochemical diagnosis of tumors. NANOG is expressed in germ cells of the fetus and in some germ cell tumors of the gonads and central nervous system (CNS).

특이성

Antibody recognizes Nanog.

면역원

Epitope: N-terminus

Synthetic peptide from mouse Nanog.

애플리케이션

Detect Nanog using this Anti-Nanog Antibody, N-terminus validated for use in WB.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Western blotting:
0.5-1.0 µg/mL. The antibody recognizes a protein of ~35 kDa on NT2/D1 cell lysate. Additional bands of ~55 and ~130 kDa may be seen depending on sample and antibody dilution used.Optimal working dilutions must be determined by the end user.

품질

Routinely evaluated by Western Blot on Embryonic Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected NANOG on 10 μg of Embryonic Mouse Brain lysates

표적 설명

39 kDa (MW 34 kDa)

물리적 형태

Affinity purified

Purified rabbit polyclonal immunoglobulin in buffer containing liquid 0.1% sodium azide.

저장 및 안정성

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

분석 메모

Control
Whole cell lysate from mouse embryonic stem cells.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.