추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse (high degree), rat, bovine, chicken, human
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... GAP43(2596)
일반 설명
GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.
특이성
Reacts to a lesser degree with rat, human, bovine and chicken. Other species have not been tested.
Recognizes Growth Associated Protein-43 (GAP-43).
면역원
Recombinant rat GAP-43 (complete sequence).
애플리케이션
Anti-Growth Associated Protein-43 (GAP-43) Antibody is an antibody against Growth Associated Protein-43 (GAP-43) for use in ELISA, IC, IH, IP & WB.
Immunohistochemistry:
1:1,000 dilution of a previous lot was used on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.
Immunocytochemistry:
1:1,000 dilution of a previous lot was used on Hippocampal neurons in culture and PC12 cells stimulated with NGF.
Immunoprecipitation:
1:100 dilution of a previous lot was used.
ELISA:
1:2,000 dilution of a previous lot was used.
1:1,000 dilution of a previous lot was used on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.
Immunocytochemistry:
1:1,000 dilution of a previous lot was used on Hippocampal neurons in culture and PC12 cells stimulated with NGF.
Immunoprecipitation:
1:100 dilution of a previous lot was used.
ELISA:
1:2,000 dilution of a previous lot was used.
품질
Routinely evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected Growth Associated Protein 43 on 10 μg of Mouse brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected Growth Associated Protein 43 on 10 μg of Mouse brain lysates.
표적 설명
23.6 kDa
물리적 형태
Format: Purified
Purified rabbit polyclonal in buffer containing PBS with 0.01% sodium azide as a preservative.
저장 및 안정성
Stable for up to 6 months at -20ºC in undiluted aliquots from date of receipt.
Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Avoid repeated freeze/thaw cycles. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
분석 메모
Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons.
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A single exposure to bisphenol A alters the levels of important neuroproteins in adult male and female mice.
Henrik Viberg,Iwa Lee
Neurotoxicology null
Exposure to polybrominated diphenyl ethers 203 and 206 during the neonatal brain growth spurt affects proteins important for normal neurodevelopment in mice.
Henrik Viberg, H Viberg
Toxicological Sciences null
Mouse glioma gene expression profiling identifies novel human glioma-associated genes.
David H Gutmann, Zhi-yong Huang, Nicole M Hedrick, Hao Ding, Abhijit Guha, Mark A Watson
Annals of Neurology null
LacZ-expressing olfactory ensheathing cells do not associate with myelinated axons after implantation into the compressed spinal cord.
Boyd, J G, et al.
Proceedings of the National Academy of Sciences of the USA, 101, 2162-2166 (2004)
Cell adhesion molecule L1 promotes neurite outgrowth of septal neurons.
Frappe, Isabelle, et al.
Journal of Neuroscience Research, 75, 667-677 (2004)
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