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Merck
모든 사진(1)

주요 문서

AB5016

Sigma-Aldrich

Anti-GABA (No Gluteraldehyde) Antibody

Chemicon®, from rabbit

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

rat

제조업체/상표

Chemicon®

기술

immunohistochemistry: suitable

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

특이성

GABA (gamma-aminobutyrate). The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, L-threonine, L-tryptophan, L-tyrosine.

면역원

GABA-glutaraldehyde

애플리케이션

Immunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.05% - 2.5% glutaraldehyde for optimum detection.

*This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative ). The minimum glutaraldehyde concentration for AB5016 is 0.00%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.

Optimal working dilutions must be determined by the end user.

DILUTION: Prepare enough of the AB5016 for your days use by diluting 100X with 1% GSPBT.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-GABA (No Gluteraldehyde) Antibody is validated for use in IH for the detection of GABA (No Gluteraldehyde).

물리적 형태

Format: Purified
IgG fraction in sterile 0.1M phosphate buffer. No preservative

저장 및 안정성

Maintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not freeze this stock.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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문서 라이브러리 방문

Matthias Braun et al.
The Journal of general physiology, 123(3), 191-204 (2004-02-11)
We have explored whether gamma-aminobutyric acid (GABA) is released by regulated exocytosis of GABA-containing synaptic-like microvesicles (SLMVs) in insulin-releasing rat pancreatic beta-cells. To this end, beta-cells were engineered to express GABA(A)-receptor Cl(-)-channels at high density using adenoviral infection. Electron microscopy
Christophe Anjard et al.
Development (Cambridge, England), 136(5), 803-812 (2009-01-30)
Encapsulation of prespore cells of Dictyostelium discoideum is controlled by several intercellular signals to ensure appropriate timing during fruiting body formation. Acyl-CoA-binding protein, AcbA, is secreted by prespore cells and processed by the prestalk protease TagC to form the 34
Haijie Yu et al.
Journal of pineal research, 61(1), 69-81 (2016-03-29)
Pinealocytes secrete melatonin at night in response to norepinephrine released from sympathetic nerve terminals in the pineal gland. The gland also contains many other neurotransmitters whose cellular disposition, activity, and relevance to pineal function are not understood. Here, we clarify
Neurotransmitter synthesis in poststroke cortical neurogenesis in adult rats.
Gu, W; Gu, C; Jiang, W; Wester, P
Stem Cell Research null
Age-dependent disease expression determines remodeling of the retinal mosaic in carriers of RPGR exon ORF15 mutations.
Beltran, WA; Acland, GM; Aguirre, GD
Investigative Ophthalmology & Visual Science null

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