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Merck
모든 사진(3)

Key Documents

AB356482

Sigma-Aldrich

Anti-mCherry

from rabbit

동의어(들):

Anti-mCherry Antibody

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성(상동성에 의해 예측)

all

기술

immunofluorescence: suitable
western blot: suitable

동형

IgG

UniProt 수납 번호

배송 상태

ambient

타겟 번역 후 변형

unmodified

일반 설명

mCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of Discosoma species. DsRed is similar in size and other properties to GFP, but it produces a red rather than a green fluorescence. mCherry is a monomeric form that has been engineered with multiple cycles of mutation, directed modification and evolutionary selection. It exhibits excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry displays high photostability and resistant to photobleaching. It also displays improved brightness and extremely rapid maturation rate. Anti-mCherry, polyclonal is an affinity purified antibody that recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref.: Schaner, NC et al. (2004). Nat. Biotechnol. 22(12):1567-72; Gross, LA et al. (2000). Proc. Natl. Acad. Sci. USA. 97(22):11990-95).

특이성

This polyclonal antibody detects mCherry in HEK293 cells tranfected with pFin-mCherry.

면역원

Epitope: unknown
His-tagged full length recombinant mCherry from Discosoma sp (Mushroom anemone).

애플리케이션

Detect mCherry protein using this rabbit polyclonal anti-mCherry Antibody, Cat. No. AB356482. It has been tested in Immunofluorescence and Western Blotting.
Immunofluorescence Analysis: A 1:500 dilution from a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vector. (Courtesy of Dr Gerry Shaw).

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vector. (Courtesy of Dr Gerry Shaw).
Research Category
Secondary & Control Antibodies

품질

Evaluated by Western Blotting in HEK293 cells transfected with pFin-EF1-mCherry vector.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected mCherry in 10 µg lysate from HEK293 cells transfected with pFin-EF1-mCherry vector.

표적 설명

~32 kDa observed, 26.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Affinity Purified
Format: Purified
Purified rabbit polyclonal antibody in PBS with 0.03% sodium azide and 50% glycerol.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


시험 성적서(COA)

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