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Merck
모든 사진(1)

주요 문서

AB3085

Sigma-Aldrich

Anti-Na+/H+ Exchanger-3 Antibody

Chemicon®, from rabbit

동의어(들):

NHE3

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity purified immunoglobulin

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

rat

제조업체/상표

Chemicon®

기술

ELISA: suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

타겟 번역 후 변형

unmodified

유전자 정보

특이성

Recognizes NHE3.
The immunogen sequence shares 90% homology with mouse NHE3.

면역원

A 22 AA peptide within the cytoplasmic, c-terminal domain of the rat NHE3 (Brant et al. 1995; Orlowsji et al. 1992; Chris Yun et al. 1995; Josette & Pouyssegur 1995). Control peptide is available, cat# AG779.

애플리케이션

Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
This Anti-Na+/H+ Exchanger-3 Antibody is validated for use in ELISA, WB for the detection of Na+/H+ Exchanger-3.
Western blot: 1-10 μg/mL using Chemiluminescence technique. A band of approximately 90 kD has been detected in brush border membrane (Yohsioka et al. 1997). ELISA: 1:100,000 using 50-100 ng control peptide/well.

Optimal working dilutions must be determined by end user.

표적 설명

93 kDa

물리적 형태

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
ImmunoAffinity Purified

저장 및 안정성

Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

분석 메모

Control
Kidney, jejunum, colon, and stomach tissue

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Jeffrey Weiss et al.
Endocrinology, 149(12), 6198-6206 (2008-08-23)
The estrogen receptor-alpha (ERalpha) acts through multiple pathways, including estrogen response element (ERE)-dependent (classical) and ERE-independent (nonclassical) mechanisms. We previously created a mouse model harboring a two-amino-acid mutation of the DNA-binding domain (E207A, G208A) that precludes direct binding of ERalpha
Differential traffic of proximal tubule Na+ transporters during hypertension or PTH: NHE3 to base of microvilli vs. NaPi2 to endosomes.
Yang, LE; Maunsbach, AB; Leong, PK; McDonough, AA
American Journal of Physiology: Renal Physiology null
Alena Cherezova et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(2), 2156-2170 (2018-09-27)
cAMP is a universal second messenger regulating a plethora of processes in the kidney. Two downstream effectors of cAMP are PKA and exchange protein directly activated by cAMP (Epac), which, unlike PKA, is often linked to elevation of [Ca2+]i. While
Evidence for NHE3-mediated Na transport in sheep and bovine forestomach.
Rabbani, I; Siegling-Vlitakis, C; Noci, B; Martens, H
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology null
Phenol injury-induced hypertension stimulates proximal tubule Na+/H+ exchanger activity.
Leong, PK; Yang, LE; Landon, CS; McDonough, AA; Yip, KP
American Journal of Physiology: Renal Physiology null

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