추천 제품
생물학적 소스
rabbit
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
bovine
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
radioimmunoassay: suitable
western blot: suitable
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
일반 설명
Fibronectin (FN) is an extracellular adhesion molecule and it is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion.
Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. FN also can serve to organize cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound FN receptors on cell surfaces. The importance of fibronectin in cell migration events during embryogenesis has been documented in several contexts, e.g.: 1) mesodermal cell migration during gastrulation can be blocked by injection of Arg-Gly-Asp (RGD) tripeptides that block cellular FN receptors (integrins); 2) injection of anti-FN antibodies into chick embryos blocks migration of precardiac cells to the embryonic midline, and; 3) the patterns of FN deposition in developing vertebrate limbs determines the patterns of precartilage cell adhesion to the ECM, thereby specifying limb-specific patterns of chondrogenesis. {D. Marcey, http://www.clunet.edu/BioDev/omm/fibro/frames/fibrotxt.htm}.
Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. FN also can serve to organize cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound FN receptors on cell surfaces. The importance of fibronectin in cell migration events during embryogenesis has been documented in several contexts, e.g.: 1) mesodermal cell migration during gastrulation can be blocked by injection of Arg-Gly-Asp (RGD) tripeptides that block cellular FN receptors (integrins); 2) injection of anti-FN antibodies into chick embryos blocks migration of precardiac cells to the embryonic midline, and; 3) the patterns of FN deposition in developing vertebrate limbs determines the patterns of precartilage cell adhesion to the ECM, thereby specifying limb-specific patterns of chondrogenesis. {D. Marcey, http://www.clunet.edu/BioDev/omm/fibro/frames/fibrotxt.htm}.
특이성
Antibody reacts with bovine fibronectin, and demonstrates cross-reactivity of less than 0.1% with bovine collagens types I, III, IV by RIA at 1:10,000 dilution.
면역원
Fibronectin extracted and purified from bovine plasma.
애플리케이션
Anti-Fibronectin Antibody detects level of Fibronectin & has been published & validated for use in ELISA, IF, RIA, WB, IH(P).
Immunohistochemistry: 1:80 dilution for immunofluorescent staining of fresh frozen bovine skin and liver tissues. Acetone or methyl-carnoy fixation is also reactive.
Immunohistochemistry on paraffin embedded tissues requires light fixation in 2% PFA, 4% formalin (less than 90 minutes), acetone or methyl-carnoy fixation; traditional formalin fixation is not recommended. Antigen retrieval is HIER citrate buffer; detection is via enhanced enzymatic methods only.
Immunoblotting: 1:1000 of 2% deoxycholate + 10% SDS, 6M urea extracted bovine cell cultures (Kinsella, 2000). Antibody demonstates the appropriate twin bands at ~220kDa.
Radioimmunoassay
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry on paraffin embedded tissues requires light fixation in 2% PFA, 4% formalin (less than 90 minutes), acetone or methyl-carnoy fixation; traditional formalin fixation is not recommended. Antigen retrieval is HIER citrate buffer; detection is via enhanced enzymatic methods only.
Immunoblotting: 1:1000 of 2% deoxycholate + 10% SDS, 6M urea extracted bovine cell cultures (Kinsella, 2000). Antibody demonstates the appropriate twin bands at ~220kDa.
Radioimmunoassay
ELISA
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
물리적 형태
Format: Purified
Protein G affinity purified IgG fraction at 1.0 mg/mL in liquid PBS (0.01M phosphate, 0.09M NaCl) pH 7.2 with no preservatives.
저장 및 안정성
Maintain frozen at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
The relationship between the nanostructure of titanium surfaces and bacterial attachment.
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Biomaterials null
Fabrication of a cell-adhesive protein imprinting surface with an artificial cell membrane structure for cell capturing.
Fukazawa K, Ishihara K
Biosensors And Bioelectronics null
Enhanced chondrocyte densities on carbon nanotube composites: the combined role of nanosurface roughness and electrical stimulation.
Dongwoo Khang, Grace E Park, Thomas J Webster
Journal of Biomedical Materials Research Part A null
Understanding greater cardiomyocyte functions on aligned compared to random carbon nanofibers in PLGA.
Asiri, AM; Marwani, HM; Khan, SB; Webster, TJ
International journal of nanomedicine null
Enhanced fibronectin adsorption on carbon nanotube/poly(carbonate) urethane: independent role of surface nano-roughness and associated surface energy.
Dongwoo Khang,Sung Yeol Kim,Peishan Liu-Snyder,G Tayhas R Palmore,Stephen M Durbin,Thomas J Webster
Biomaterials null
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