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생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, human, rat
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
특이성
Recognizes Delta opioid receptor. The control peptide (catalog number AG288) is available for use in antibody blocking studies.
면역원
Delta opioid receptor N-terminal peptide (LVPSARAELQSSPLV).
Epitope: N-terminus
애플리케이션
Anti-Opioid Receptor Antibody, δ, pain, N-terminus detects level of Opioid Receptor & has been published & validated for use in FC, IH(P) & WB.
Immunohistochemistry on CNS tissues fixed in PLP: 1:1,000.
Reactive on paraffin embedded tissue.
Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.
FACS on human T-cells (Sharp, 2001)
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (without Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Reactive on paraffin embedded tissue.
Western Blot: 1:1000. Under reducing conditions the antibody will detect major bands at 36 and 72 kDa.
FACS on human T-cells (Sharp, 2001)
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1560
This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.
1) 3 x 10 minute washes in TBS (without Triton).
2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).
3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (without Triton) for 2 hours at room temperature followed by 16 hours at 4°C.
4) 3 x 10 minute washes in TBS.
5) Incubate with secondary antibody diluted appropriately in TBS with1% serum (same as host from secondary antibody).
6) 3 x 10 minute washes in TBS.
7) ABC Elite (1:200 Vector Labs) in TBS.
8) 2 x 10 minute washes in TBS.
9) 1 x 10 minute wash in phosphate buffer (no saline).
10) DAB reaction with 0.06% NiCl added for intensification.
11) 2 x 10 minute washes in PBS.
12) 1 x 10 minute wash in phosphate buffer (no saline).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuroinflammation & Pain
Neuroinflammation & Pain
표적 설명
36 and 72 kDa
결합
Replaces: MAB5330
물리적 형태
UnPurified whole rabbit antiSerum containing no preservatives.
Unpurified
저장 및 안정성
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Spinal cord
Spinal cord
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Comparison of immunoblotted delta opioid receptor proteins expressed in the adult rat brain and their regulation by growth hormone.
Persson, Anders I, et al.
Neuroscience Research, 52, 1-9 (2005)
Peripheral ?-opioid receptors attenuate the exercise pressor reflex.
Leal, AK; Yamauchi, K; Kim, J; Ruiz-Velasco, V; Kaufman, MP
American Journal of Physiology. Heart and Circulatory Physiology null
Glial-restricted precursors: patterns of expression of opioid receptors and relationship to human immunodeficiency virus-1 Tat and morphine susceptibility in vitro.
S K Buch, V K Khurdayan, S E Lutz, P E Knapp, N El-Hage, K F Hauser
Neuroscience null
Yuan Xu et al.
Aging, 12(24), 25035-25059 (2020-11-17)
Our previous studies have shown that the δ-opioid receptor (DOR) is an important neuroprotector via the regulation of PTEN-induced kinase 1 (PINK1), a mitochondria-related molecule, under hypoxic and MPP+ insults. Since mitochondrial dysfunctions are observed in both hypoxia and MPP+
Noufissa Kabli et al.
Pain, 127(1-2), 84-93 (2006-09-12)
The analgesic effects of local administration of opioid agonists into peripheral tissues in alleviating pain have been well documented in both clinical and preclinical studies, although few studies have examined their effects in neuropathic pain. In this study, we investigated
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