추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
pig, bovine, canine, human
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ADORA2A(135)
특이성
Adenosine A2a receptor. Recognizes a band of approximately 45 kDa in human (coronary artery), and rat, porcine and bovine striatal membranes.
면역원
Amino acid sequence near the 4th C-terminal intracellular domain (30 amino acids) of the cloned canine A2a receptor. The A2a sequence selected is not present in A1, A2b and A3 receptors. Control peptide is available for this product (Catalog Number AG285).
애플리케이션
Anti-Adenosine A2a Receptor Antibody is an antibody against Adenosine A2a Receptor for use in ELISA, IC, IH & WB.
Immunohistochemistry: 10-50 μg/mL on frozen tissue sections
Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG285)/well)
Optimal working dilutions must be determined by end user.
Western blot: 1-10 μg/mL (Chemiluminescence technique), 40-100 μg membrane proteins/lane of striatal membranes. Higher concentrations of the antibody may be necessary if colorimetric detection or milk-based antibody diluent are used.
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
ELISA: 1:10,000-1:50,000 (50-100 ng A2aR peptide (Cat. Number AG285)/well)
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
물리적 형태
Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA and 0.05% sodium azide.
저장 및 안정성
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
A L Tucker et al.
Cardiovascular research, 27(1), 62-67 (1993-01-01)
Four subtypes of adenosine receptors have recently been cloned from thyroid, brain and testis. In this review we have summarised properties of these purinergic receptors. The cloned A1 and A2 subtypes are probably similar or identical to receptors that exist
R B Marala et al.
The Journal of pharmacology and experimental therapeutics, 286(2), 1051-1057 (1998-08-08)
Antipeptide antibody was raised in rabbit against the sequence (361-390) of RDC-8, the presumed adenosine A2A receptor cDNA from canine. The antibody titer was estimated by solid phase radioimmunoassay. Western blot analysis under reducing conditions identified a major 45 +/-
Expression of adenosine receptors in the preglomerular microcirculation.
Edwin K Jackson, Chongxue Zhu, Stevan P Tofovic
American Journal of Physiology: Renal Physiology null
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