AB15570-I

Anti-Tryptophan hydroxylase 1/TPH1 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse
• 종 반응성(상동성에 의해 예측): rat (based on 100% sequence homology)
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI 수납 번호: NP_001094104
• UniProt 수납 번호: P09810
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Tph1(21990) ; rat ... Tph1(24848)

설명

일반 설명

Tryptophan hydroxylase 1/TPH1, also known as Tryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, and encoded by the gene TPH1/TPH/TPRH/TRPH, is an important biosynthetic enzyme needed for serotonin synthesis from L-trytophan. TPH1 is responsible for the addition of the -OH grouip to 5-hydroxytryptophan which is the initial and rate limiting step in the synthesis of serotonin. Serotonin in a neurotransmitter and has an antidepressant effect on the brain. The activity of TPH1 is also modulated by phosphorylation Protein Kinase A is responsible for the phosphorylation of TPH1 and helps to modulate its activity in neurons. TPH1 is mostly expressed in tissues that express serotonin such as the brain, pineal gland, and CNS but also can be found in skin and intestinal tissues.

면역원

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of rat Tryptophan hydroxylase 1/TPH1.

애플리케이션

Immunohistochemistry Analysis: A 1:50-500 dilution from a representative lot detected Tryptophan hydroxylase 1/TPH1 in mouse pancreas and mouse colon tissue.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Tryptophan hydroxylase 1/TPH1 in mouse cerebellum and mouse cerebral cortex tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This Anti-Tryptophan hydroxylase 1/TPH1 antibody is validated for use in western blotting & IHC (Paraffin) for the detection of Tryptophan hydroxylase 1/TPH1.

품질

Evaluated by Western Blotting in P815 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Tryptophan hydroxylase 1/TPH1 in 10 µg of P815 cell lysate.

표적 설명

~51 kDa observed

결합

Replaces: AB15570

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable