69839-M

T4 DNA Ligase - Novagen

Qualified under the most stringent cloning conditions

• 동의어(들): DNA Ligation
• UNSPSC 코드: 41106310
• NACRES: NA.54

속성

• Quality Level: 200
• 제조업체/상표: Novagen^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 배송 상태: dry ice; wet ice
• 저장 온도: −20°C

설명

일반 설명

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 3′-hydroxyl and 5′-phosphate groups of adjacent DNA and RNA nucleotides in blunt-end or cohesive-end configurations. The enzyme joins RNA and DNA strands only in duplex molecules and does not join single-stranded nucleic acids. Novagen T4 DNA Ligase is rigorously tested in a blue-white cloning assay under conditions that maximize exposure of DNA ends to any contaminating nucleases. The enzyme is qualified for any ligation or cloning application.

경고

Toxicity: Standard Handling

단위 정의

4 Weiss units/µl; one Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of ³²P from pyrophosphate into ATP as Norit^®-absorbable material in 20 minutes at 37°C. 0.01 Weiss unit is the amount of enzyme required to ligate > 95% of 1 µg bacteriophage λ Hind III fragments at 16°C in 20 minutes.

법적 정보

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Norit is a registered trademark of Norit N.V.

안전성 정보

• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable