567333

SNAPtide Botulinum Toxin A Substrate, Fluorogenic

≥90% (HPLC), lyophilized, FRET substrate, Calbiochem^®

• 동의어(들): Botulinum Toxin Assay
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• product name: SNAPtide Botulinum Toxin A Substrate, Fluorogenic, SNAPtide Botulinum Toxin A Substrate, Fluorogenic, is a synthetic peptide that acts as a FRET substrate with the fluorophore o-aminobenzoic acid and the acceptor chromophore 2,4-dinitrophenol (DNP).
• Quality Level: 100
• 분석: ≥90% (HPLC)
• 형태: lyophilized
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 형광: λ_ex ~326 nm; λ_em ~423 nm
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

A synthetic peptide, fluorescence resonance energy transfer (FRET) substrate containing the N-terminally-linked fluorophore o-aminobenzoic acid (Abz) and the acceptor chromophore 2,4-dinitrophenol (DNP) linked to the C-terminus. The substrate is based on the native botulinum toxin type A cleavage site found in the synaptosome-associated protein SNAP-25. Cleavage of the substrate by botulinum toxin releases the fluorophore and full fluorescence is restored.

생화학적/생리학적 작용

Cell permeable: no

Primary Target
Substrate for the native botulinum toxin

Product does not compete with ATP.

Reversible: no

경고

Toxicity: Standard Handling (A)

물리적 형태

Lyophilized from water.

제조 메모

Prepare a 5 mM stock solution of this peptide in DMSO as follows: Add 40 µl of DMSO to a vial containing 200 nmol of peptide.

재구성

Following reconstitution, aliquot, cover with foil to protect from light, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

기타 정보

Bigalke, H. and Shoer, L.F. 2000. In: Handbook of Experimental Pharmacology,145, Bacterial Protein Toxins (K. Aktories and I. Just, Eds.) pp. 407-443, Springer-Verlag, Berlin.
Schiavo, G., et al. 1993. J. Biol. Chem.268, 23784.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable