553514-M

RAD51 Inhibitor II, RI-1

The RAD51 Inhibitor, RI-1 controls the biological activity of RAD51. This small molecule/inhibitor is primarily used for Cell Structure applications.

• 동의어(들): RAD51 Inhibitor II, RI-1, 3-chloro-1-(3,4-dichlorophenyl)-4-morpholino-1H-pyrrole-2,5-dione
• 실험식(Hill 표기법): C₁₄H₁₁Cl₃N₂O₃
• Molecular Weight: 361.61
• UNSPSC 코드: 12352200

속성

• Quality Level: 100
• 분석: ≥99% (HPLC)
• 양식: solid
• 효능: 5-30 μM IC₅₀
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; protect from light
• 색상: bright yellow
• solubility: DMSO: 50 mg/mL
• 저장 온도: 2-8°C

설명

일반 설명

A cell-permeable 1H-Pyrrole-2,3-dione derivative that covalently binds to RAD51 at Cys319 and irreversibly destabilizes a protein-protein interface that is essential for filament formation and recombinase activity. Stimulates single strand annealing at a 5-30 µM concentration. Inhibits subnuclear RAD51 foci formation following DNA damage without affecting replication protein A. Also, effectively sensitizes cancer cells to mitomycin C (~25 µM) and generate synergistic cell kill in RAD51C mutant cells and with lesser effect on RAD51 complemented cells. Satisfies all four Lipinski rules for predicting drug likeness.

생화학적/생리학적 작용

Primary Target
RAD51

경고

Toxicity: Standard Handling (A)

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

기타 정보

Budke, B., et al. 2012. Nucleic Acids Res.40, 7347.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable