529659

DDD00033325

≥95% (HPLC), syrup, single hESC survival promoter, Calbiochem^®

• 동의어(들): Pro-survival Compound, DDD00033325, DDD00033325
• 실험식(Hill 표기법): C₁₇H₁₉N₅
• CAS Number: 1259034-82-6
• Molecular Weight: 293.37
• UNSPSC 코드: 51111800
• NACRES: NA.77

속성

• product name: Pro-survival Compound, DDD00033325, A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background with equal or higher potency than Y-27632.
• Quality Level: 100
• 분석: ≥95% (HPLC)
• 형태: syrup
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; protect from light
• 색상: yellow
• solubility: DMSO: 100 mg/mL
• 배송 상태: wet ice
• 저장 온도: −20°C
• SMILES string: C12=CC=NN1C=CC(N[C@@H]3CCN(CC4=CC=CC=C4)C3)=N2

설명

일반 설명

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 (Cat. No. 688000) in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC₅₀ = 3.9 M), with equal or higher potency than Y-27632 in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 M. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 M. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 M than Y-27632 without marked adverse effects for up to 120 hours.

포장

Packaged under inert gas

경고

Toxicity: Regulatory Review (Z)

재구성

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

기타 정보

Andrews, P.D., et al. 2010. Biochem. J.432, 21.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable