추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
PY20, monoclonal
양식
liquid
포함
0.1% Proclin as preservative
종 반응성(상동성에 의해 예측)
all
제조업체/상표
Calbiochem®
저장 조건
OK to freeze
동형
IgG2b
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
phosphorylation (pTyr)
유전자 정보
human ... PID1(55022)
일반 설명
Peroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.
면역원
KLH conjugated to phosphotyrosine, glycine, and alanine
애플리케이션
ELISA (1:2000)
Immunoblotting (1:500-1:2000)
Immunohistochemistry (1:500)
Immunoblotting (1:500-1:2000)
Immunohistochemistry (1:500)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
물리적 형태
In PBS, 50% glycerol, 1% BSA, pH 7.4.
기타 정보
Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Specific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with <150 mM NaCl and divalent cations not much >1 mM because this antibody′s binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Andrew J Garton et al.
Cancer research, 66(2), 1015-1024 (2006-01-21)
OSI-930 is a novel inhibitor of the receptor tyrosine kinases Kit and kinase insert domain receptor (KDR), which is currently being evaluated in clinical studies. OSI-930 selectively inhibits Kit and KDR with similar potency in intact cells and also inhibits
Justin M Scheer et al.
PloS one, 7(12), e51817-e51817 (2013-01-04)
The structure of the Fab region of antibodies is critical to their function. By introducing single cysteine substitutions into various positions of the heavy and light chains of the Fab region of trastuzumab, a potent antagonist of HER2, and using
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