524715

Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda

• 동의어(들): PDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 양식: liquid
• Quality Level: 100
• 특이 활성도: ≥50,000 units/mL
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 배송 상태: wet ice
• 저장 온도: −70°C

설명

일반 설명

Recombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583).

Recombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583). For enzymatic studies a dilution of 1:1000 recommended. For use as a immunoblot standard 2 to 3 µl per lane is recommended. In immunoblotting, gives band at ~92 kDa.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.

물리적 형태

In sodium phosphate buffer, 20% glycerol, pH 7.2.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1