5.08838

Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested

• 동의어(들): Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested, (2 S)-2-Amino-N-((2 S,3 S,4 R,5 R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)
• 실험식(Hill 표기법): C₂₂H₂₉N₇O₅ · 2HCl
• CAS Number: 58-58-2
• Molecular Weight: 544.43
• MDL number: MFCD00012691
• UNSPSC 코드: 12352101

속성

• 분석: ≥98% (HPLC)
• Quality Level: 200
• 형태: liquid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• 불순물: ≤10 EU/mg Endotoxin (dry weight)
• 저장 온도: −20°C
• InChI: 1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16?,18+,22+;;/m0../s1
• InChI key: MKSVFGKWZLUTTO-USYAMCSGSA-N

설명

일반 설명

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor.

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.

생화학적/생리학적 작용

Primary Target
RNA

Reversible: yes

경고

Toxicity: Standard Handling (A)

물리적 형태

Supplied at 10 mg/ml in H₂O.

재구성

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

기타 정보

Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Kaufman, S.H., et al. 1993. Cancer Res.53, 3976.
de la Luna, S. and Ortin, J. 1992. Methods Enzymol.216, 376.
Chow, S.C., et al. 1991. Exp. Cell Res.216, 149.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°C): Not applicable