444232

MMP-9, Dimer, Human Neutrophil

• 동의어(들): Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase
• 효소 위원회 번호: 3.4.24.35 (BRENDA, IUBMB)
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 분석: ≥90% (SDS-PAGE)
• Quality Level: 100
• 형태: liquid
• 특이 활성도: ≥1000 mU/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 외래 활성: other MMP activity, none detected
• 배송 상태: wet ice
• 저장 온도: −70°C

설명

일반 설명

Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.

M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.

물리적 형태

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

제조 메모

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Olson, M.W. et al. 2000. J. Biol. Chem.275, 2661.
Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

법적 정보

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable