440319

Lumican, His•Tag^®, CF, Mouse, Recombinant

• UNSPSC 코드: 12352203
• NACRES: NA.45

속성

• 분석: ≥95% (SDS-PAGE)
• Quality Level: 100
• 형태: lyophilized
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 불순물: ≤1.0 EU/μg protein Endotoxin
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Recombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.

생화학적/생리학적 작용

Cell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 10⁴ BCE C/D-1b bovine cornea-derived endothelial cells.

경고

Toxicity: Standard Handling (A)

물리적 형태

Lyophilized from 0.2 µm filtered PBS.

재구성

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 200 µg/ml with sterile PBS.

기타 정보

D′Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun.365, 266.
Nikitovic, D. et al. 2008. IUBMB Life60, 818.
Nikitovic, D. et al. 2008. FEBS J.275, 350.
Ishiwata, T. et al. 2007. Oncol. Rep.18, 537.
Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294.
Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422.
Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532.
Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365.
Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347.
Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2