407707

PhosphoDetect Anti-Insulin Receptor (pTyr^1162/1163) Rabbit pAb

liquid, Calbiochem^®

• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: liquid
• 포함: ≤0.1% sodium azide as preservative
• 종 반응성: human
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 동형: IgG
• 배송 상태: wet ice
• 저장 온도: −70°C
• 타겟 번역 후 변형: phosphorylation (pTyr1162/pTyr1163)
• 유전자 정보: human ... INSR(3643)

설명

일반 설명

Note: 1 T = 1 test. Sufficient for 10 Western mini blots.

Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the insulin receptor (IR) protein phosphorylated at Tyr^1162/1163 and insulin-like growth factor 1 receptor (IGF-1R) protein phosphorylated at Tyr^1162/1163.

Recognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr¹¹⁶² and Tyr¹¹⁶³ in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr¹¹³⁵ and Tyr¹¹³⁶.

This PhosphoDetect Anti-Insulin Receptor (pTyr^1162/1163) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr^1162/1163).

면역원

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^1162/1163 phosphorylation sites of human insulin receptor

애플리케이션

ELISA (see comments)

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

경고

Toxicity: Standard Handling (A)

재구성

Following initial thaw, aliquot and freeze (-20°C).

기타 정보

Bevan, P. 2001. J. Cell Sci.114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest.103, 931.
Schmid, E., at al. 1998. FASEB J.12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.
Ebina, Y., et al. 1985. Cell40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation27, 83.
Grunberger, G., et al. 1984. Science223, 932.

The immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable