393207-M

Anti-HNE-Michael Adducts, Reduced Rabbit pAb

liquid, Calbiochem^®

• 동의어(들): Anti-4-Hydroxy-2-noneal Michael Adducts, Reduced
• UNSPSC 코드: 12352203

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: serum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: liquid
• 미포함: preservative
• 종 반응성(상동성에 의해 예측): all
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 동형: IgG
• 저장 온도: −70°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Anti-HNE-Michael Adducts, Reduced, rabbit polyclonal, specifically recognizes chemically reduced amino acid-(4)-HNE adducts. It is validated for use in ELISA, WB & IHC (frozen & paraffin sections).

Rabbit polyclonal antibody supplied as diluted serum. Recognizes, specifically, chemically reduced amino acid-(4)-HNE adducts.

Recognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such as trans-2-noneal.

The CNS is enriched in polyunsaturated fatty acids and is therefore particularly vulnerable to lipid peroxidation. 4-Hydroxy-2-noneal (HNE) is one of the major lipid peroxidation products with cytotoxic and mutagenic activity. It reacts with protein residues such as histidine to generate stable Michael adducts. HNE has been shown to inhibit neurite outgrowth and disrupt microtubules and tubulin modification, suggesting it may play a role in Alzheimer′s disease.

특이성

A broad range of species

면역원

HNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced

애플리케이션

ELISA (1:500-1:1000)

Immunoblotting (1:1000-1:4000)

Frozen Sections (1:50-1:500)

Paraffin Sections (1:50-1:500)

경고

Toxicity: Standard Handling (A)

물리적 형태

Serum diluted in 50% glycerol, pH 7.5.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Does not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.

Toyokuni, S., et al. 2000. Antioxid. Redox. Signal2, 681.
Neely, M.D., et al. 1999. J. Neurochem.72, 2323.
Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun.212, 1068.
Uchida, K., et al. 1995. Arch. Biochem. Biophys.324, 241.
Uchida, K., et al. 1994. Biochemistry33, 12487.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1