371206

Dkk-1, His•Tag^®, Mouse, Recombinant

Dkk-1, His•Tag, Mouse, Recombinant, consists of amino acids 30-272, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-dervied mouse myeloma cells.

• UNSPSC 코드: 12352203

속성

• 재조합: expressed in mouse NSO cells (mouse myeloma cells)
• Quality Level: 100
• 분석: ≥95% (SDS-PAGE)
• 형태: lyophilized
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 불순물: ≤1.0 EU/μg protein Endotoxin
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Dkk-1, His•Tag, Mouse, Recombinant, consists of amino acids 30-272, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-dervied mouse myeloma cells.

Recombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.

생화학적/생리학적 작용

ND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.

Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.

경고

Toxicity: Regulatory Review (Z)

물리적 형태

Lyophilized from 0.2 µm filtered PBS, BSA.

재구성

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

기타 정보

Pinzone, J.J. et al. 2009. Blood113, 517.
Li, J. et al. 2006. Bone36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C. 2006. Oncogene25, 7469.
Verani, R. et al. 2006. J. Neurochem.101, 242.
Cappuccio, I. et al. 2005. J. Neurosci.25, 2647.
Kemp, C. et al. 2005. Dev. Dyn.233, 1064.
Adamska, M. et al. 2004. Dev. Biol.272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Glinka, A. et al. 1998. Nature391, 357.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable