344826

α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli

This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides.

• 동의어(들): α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli, α-L-Fucoside Fucohydrolase
• CAS Number: 9037-65-4
• 효소 위원회 번호: 3.2.1.51 (BRENDA, IUBMB)
• UNSPSC 코드: 12352202
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 형태: liquid
• 특이 활성도: ≥1.0 units/mL; ≥1.5 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: do not freeze
• 외래 활성: Proteases, none detected
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Note: 1 mU = 1 milliunit.

Recombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.

Recombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium meningosepticum) catalyses the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.

물리적 형태

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

기타 정보

Zheng, Q., et al. 2002. J. Biol. Chem.277, 39823.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable