324740

Elastase Substrate V, Fluorogenic

≥95% (HPLC), powder, lyophilized solid, elastase substrate, Calbiochem^®

• 동의어(들): Elastase Substrate V, Fluorogenic, MeOSuc-AAPV-AMC
• 실험식(Hill 표기법): C₃₁H₄₁N₅O₉
• CAS Number: 72252-90-5
• Molecular Weight: 627.69
• MDL number: MFCD00077135
• UNSPSC 코드: 12352200
• NACRES: NA.25

속성

• product name: Elastase Substrate V, Fluorogenic, Elastase Substrate V, Fluorogenic, CAS 72252-90-5, is a sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM).
• Quality Level: 200
• 분석: ≥95% (HPLC); ≥95% (TLC)
• 형태: lyophilized solid; powder
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated; protect from light
• 색상: white to off-white
• solubility: ethanol: 1 mg/mL; DMF: 5 mg/mL; DMSO: 5 mg/mL
• 형광: λ_ex 360-380 nm; λ_em 430-460 nm
• 배송 상태: ambient
• 저장 온도: −20°C
• InChI: 1S/C31H41N5O9/c1-16(2)27(34-20-9-10-21-17(3)14-26(39)45-23(21)15-20)30(42)35-29(41)22-8-7-13-36(22)31(43)19(5)33-28(40)18(4)32-24(37)11-12-25(38)44-6/h9-10,14-16,18-19,22,27,34H,7-8,11-13H2,1-6H3,(H,32,37)(H,33,40)(H,35,41,42)/t18-,19-,22-,27-/m0/s1
• InChI key: NHMQNKPYBPDCAY-NFHWZJRKSA-N

설명

일반 설명

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (K_m = 140 µM; K_cat/K_m = 120,000 s^-1 M^-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM; k_cat/K_m = 120,000 M^-1 s^-1). Not hydrolysed by cathepsin G.

생화학적/생리학적 작용

Cell permeable: no

Km = 140 µM; kcat/Km = 120,000 M⁻¹ s⁻¹as substrates for human leukocyte and porcine pancreatic elastase

Primary Target
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase

Product does not compete with ATP.

Reversible: no

경고

Toxicity: Standard Handling (A)

서열

MeOSuc-Ala-Ala-Pro-Val-AMC

재구성

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.

기타 정보

Barrett, A.J. 1981. Methods Enzymol. 80, 581.
Castillo, M.J., et al. 1979. Anal. Biochem.99, 53.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable