317640-M

Dipeptidyl Peptidase IV (CD26), Porcine Kidney

• 동의어(들): CD26, DPPIV
• 효소 위원회 번호: 3.4.14.5 (BRENDA, IUBMB)
• UNSPSC 코드: 12352202

속성

• 분석: ≥90% (SDS-PAGE)
• Quality Level: 100
• 양식: liquid
• 특이 활성도: ≥35 units/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 저장 온도: −70°C

설명

일반 설명

Native, DPP IV purified from porcine kidney (renal brush border of the proximal tubule). A serine exopeptidase dimer composed of two identical subunits of 110-130 kDa. Cleaves Xaa-Pro dipeptides from the N-terminus of oligo and polypeptides. DPPIV is involved in many cellular processes such as activation of cytokines, differentiation, T cell activation, and cell-matrix interactions. Inhibition of DPPIV has been reported to be an effective treatment for type II diabetes. MW: ~210000-260000 (unreduced).

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

단위 정의

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmole 7-(Gly-Pro)-amino-4-methylcoumarinamide per min at 37°C, pH 8.5.

물리적 형태

In 20 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% NaN₃, pH 8.0.

재구성

Dilute only the amount of enzyme needed for each assay. Upon thawing, do not re-freeze, store in the refrigerator (4°C).

기타 정보

Bar, J., et al. 2003. Biol. Chem.384, 1553.
Engel, M., et al. 2003. Proc. Natl. Acad. Sci. USA100, 5063.
Ikehara, Y., et al. 1994. Methods. Enzymol.244, 215.
David, F., et al. 1993. J. Biol. Chem.268, 17247.
Thomsen, P.D., et al. 1993. Mamm. Genome4, 604.
Misumi, Y., et al. 1992. Biochim. Biophys. Acta.1131, 333.
Seidl, R., et al. 1991. Biol. Chem. Hoppe Seyler372, 213.
Checler, F., et al. 1985. J. Neurochem.45, 1509.
Imai, K., et al. 1983. J. Biochem.93, 431.
Kato, T., et al. 1979. Experientia.35, 409.
Kojima, K., et al. 1979. Anal. Biochem.100, 43.
Kato, T., et al. 1978. Biochem. Med.19, 351.
Kenny, A.J., et al. 1976. Biochem. J.157, 169.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable