217669

Anti-CD28 Mouse mAb (ANC28.1/5D10)

liquid, >95% (immunoglobulin, SDS-PAGE), clone ANC28.1/5D10

• 동의어(들): Anti-CD28 Antibody, CD28 Detection Antibody, Mouse Anti-CD28
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: ANC28.1/5D10, monoclonal
• 분석: >95% (immunoglobulin, SDS-PAGE)
• 형태: liquid
• 포함: 500 μg/mL gentamycin sulfate as preservative
• 종 반응성: human
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze
• 동형: IgG1
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CD28(940)

설명

일반 설명

Anti-CD28, mouse monoclonal, clone ANC28.1/5D10, recognizes CD28 on human T cells & human CD28 transfected BHK cells. It is validated for FC, IP, IHC on frozen sections, and T cell co-stimulation.

Protein A purified mouse monoclonal antibody. Recognizes the CD28 protein.

Recognizes CD28 on human T cells and BHK cells transfected with human CD28.

면역원

Human

human CD28 Ig fusion protein and HPB-MLT human tumor cells

애플리케이션

Flow Cytometry (5 µg/ml)

Frozen Sections (see comments)

Immunoprecipitation (see comments)

T cell Co-stimulation (see comments)

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

물리적 형태

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

재구성

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

기타 정보

Kariv, I., et al. 1996. J. Immunol.157, 29.
61st Forum in Immunology. 1995. Res. Immunol.146, 127.
Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol.5, 361.

Monomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x10⁵ cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable