208772

Calpain-1 Substrate II, Fluorogenic

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides.

• 동의어(들): Calpain-1 Substrate II, Fluorogenic, H-E(EDANS)PLF~AERK(DABCYL)-OH
• 실험식(Hill 표기법): C₇₂H₉₇N₁₇O₁₆S
• Molecular Weight: 1488.71
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: ≥97% (HPLC)
• 형태: lyophilized solid
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; desiccated (hygroscopic); protect from light
• 색상: red
• solubility: DMSO: 5 mg/mL
• 형광: λ_ex ~505 nm; λ_em ~335 nm
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively.

An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides. Shown to be kinetically superior, in terms of turnover rate, to Calpain Substrate III, Fluorogenic (Cat. No. 208771), Calpain-1 Substrate, Fluorogenic (Cat. No. 208748), and Calpain Substrate II, Fluorogenic (Cat. No. 208731) by ~8-, 18-, and 310-fold, respectively. Excitation: ~335 nm; emission: ~505 nm.

생화학적/생리학적 작용

Cell permeable: no

Primary Target
Calpain-1 Substrate

Product does not compete with ATP.

Reversible: no

포장

Packaged under inert gas

경고

Toxicity: Irritant (B)

서열

H₂N-Glu(EDANS)-Pro-Leu-Phe~Ala-Glu-Arg-Lys(DABCYL)-CO₂H

물리적 형태

Supplied as a trifluoroacetate salt.

재구성

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

기타 정보

Cuerrier, D., et al. 2007. J. Biol. Chem.282, 9600.
Cuerrier, D., et al. 2005. J. Biol. Chem.280, 40632.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable