203195

Protein A, Biotin Conjugate

This Protein A, Biotin Conjugate is validated for use in Solid Phase Immunoassay.

• UNSPSC 코드: 41116133
• NACRES: NA.41

속성

• Quality Level: 100
• 양식: liquid
• 포함: ≤0.1% sodium azide as preservative
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 배송 상태: ambient
• 저장 온도: −20°C

설명

일반 설명

Native protein from Straphylococcus Aureus, conjugated to biotin. Recognizes the Fc portions of immunoglobulins of various species.

Native protein A conjugated to biotin.

애플리케이션

Solid Phase Immunoassay (1:10,000)

포장

Please refer to vial label for lot-specific concentration.

경고

Toxicity: Standard Handling (A)

물리적 형태

In 10 mM PBS, 1% BSA, pH 7.6.

재구성

Following initial thaw, aliquot and freeze (-20°C).

분석 메모

A single band at MW 50,000 by SDS-PAGE

기타 정보

The protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable