추천 제품
생물학적 소스
rabbit
Quality Level
클론
polyclonal
종 반응성
human
종 반응성(상동성에 의해 예측)
hamster (based on 100% sequence homology), canine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
제조업체/상표
ChIPAb+
Upstate®
기술
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI 수납 번호
배송 상태
dry ice
일반 설명
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ HDAC1 polyclonal set includes the HDAC1 antibody, a Normal Rabbit IgG, and control primers which amplify a 88 bp region of ChIP Primers p21. The HDAC1 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC1-associated chromatin.
The ChIPAb+ HDAC1 polyclonal set includes the HDAC1 antibody, a Normal Rabbit IgG, and control primers which amplify a 88 bp region of ChIP Primers p21. The HDAC1 and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of HDAC1-associated chromatin.
면역원
Synthetic peptide corresponding to amino acids 467-482 of human HDAC1.
애플리케이션
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using using 2 µg of either Normal Rabbit IgG, or 2 µg of Anti-HDAC1 and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers, p21 . (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
0.5 µg/mL from a representative lot detected HDAC1 in HeLa cells. (Figure 3).
Immunofluorescence Analysis:
2 µg/mL from a representative lot detected HDAC1 in HeLa cells.
Representative lot data.
Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using using 2 µg of either Normal Rabbit IgG, or 2 µg of Anti-HDAC1 and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers, p21 . (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
0.5 µg/mL from a representative lot detected HDAC1 in HeLa cells. (Figure 3).
Immunofluorescence Analysis:
2 µg/mL from a representative lot detected HDAC1 in HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
This ChIPAb+ HDAC1 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
포장
25 assays per set. Recommended use: ~2 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).
품질
Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from U2OS cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG, or 2 µg of Anti-HDAC1 and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers, p21 flanking an Sp1 binding site in the human p21 promoter (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Representative lot data.
Sonicated chromatin prepared from U2OS cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either Normal Rabbit IgG, or 2 µg of Anti-HDAC1 and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of HDAC1 associated DNA fragments was verified by qPCR using ChIP Primers, p21 flanking an Sp1 binding site in the human p21 promoter (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
표적 설명
~62 kDa observed
물리적 형태
Anti-HDAC1 (rabbit polyclonal). One vial containing 50 µg of Anti-HDAC1 IgG in PBS with 1 mg/mL BSA and ≤0.1% sodium azide before the addition of glycerol to 30%. Store at -20°C.
Concentration: 0.7 mg/mL
Normal Rabbit IgG. One vial containing 125 µg of rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers p21. One vial containing 75 μL of 5 μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at -20°C.
FOR: CCC ACA GCA GAG GAG AAA GAA
REV: CTG GAA ATC TCT GCC CAG ACA
Concentration: 0.7 mg/mL
Normal Rabbit IgG. One vial containing 125 µg of rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers p21. One vial containing 75 μL of 5 μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at -20°C.
FOR: CCC ACA GCA GAG GAG AAA GAA
REV: CTG GAA ATC TCT GCC CAG ACA
Format: Purified
저장 및 안정성
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
분석 메모
Control
Includes normal rabbit IgG and primers specific for human p21.
Includes normal rabbit IgG and primers specific for human p21.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Baoling Bai et al.
Birth defects research, 110(14), 1118-1128 (2018-08-17)
Maternal diabetes related neural tube defects (NTDs) are a result of oxidative stress and apoptosis. However, the molecular mechanism behind the pathogenesis is not fully understood. Here, we report that high glucose exposure-induced epigenetic changes influence histone H4 acetylation and
Jing Wang et al.
Scientific reports, 6, 39469-39469 (2016-12-21)
Hypertension is a major risk factor for cardiovascular and cerebrovascular disease. Prenatal exposure to lipopolysaccharide (LPS) leads to hypertension in a rat offspring. However, the mechanism is still unclear. This study unraveled epigenetic mechanism for this and explored the protective
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