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Merck
모든 사진(1)

주요 문서

16-101

Sigma-Aldrich

Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate

clone 4G10®, Upstate®, from mouse

동의어(들):

4G10 Phosphotyrosine Antibody, Clone 4G10 Anti-Phosphotyrosine, Phosphotyrosine Detection Antibody

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

4G10®, monoclonal

종 반응성

vertebrates

제조업체/상표

Upstate®

기술

immunoprecipitation (IP): suitable

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... PID1(55022)

일반 설명

Anti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.

특이성

Recognizes tyrosine-phosphorylated proteins from all species.

면역원

Phosphotyramine-KLH.

애플리케이션

Anti-Phosphotyrosine Antibody, clone 4G10, agarose conjugate is an antibody against Phosphotyrosine for use in IP.
Research Category
Signaling
Research Sub Category
General Post-translation Modification
This product is useful for immunoprecipitation and immunoaffinity chromatography methods.

Suitable for immunoprecipitation and affinity purification.

특징 및 장점

Format: Gel Immobilized

품질

Evaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.

Immunoprecipitation Analysis:
10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.

표적 설명

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

물리적 형태

1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% Kathon as a preservative, for a total volume of 2 mL. Liquid suspension.
Protein A purified

저장 및 안정성

Stable for 1 year at 2-8ºC from date of receipt.

분석 메모

Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Identification of oncogenic point mutations and hyperphosphorylation of anaplastic lymphoma kinase in lung cancer.
Wang, YW; Tu, PH; Lin, KT; Lin, SC; Ko, JY; Jou, YS
Neoplasia null
Activation of src-related tyrosine kinases by IL-3
Anderson, S. M. and Jorgensen, B.
Journal of immunology (Baltimore, Md. : 1950), 155, 1660-1670 (1995)
Robin White et al.
The Journal of biological chemistry, 287(3), 1742-1754 (2011-12-01)
Myelin basic protein (MBP) is a major component of central nervous system (CNS) myelin. The absence of MBP results in the loss of almost all compact myelin in the CNS. MBP mRNA is sorted into RNA granules that are transported
Heparin binding and oligomerization of hepatocyte growth factor/scatter factor isoforms. Heparan sulfate glycosaminoglycan requirement for Met binding and signaling.
Sakata, H, et al.
The Journal of Biological Chemistry, 272, 9457-9463 (1997)
Phosphorylation site dynamics of early T-cell receptor signaling.
Chylek, LA; Akimov, V; Dengjel, J; Rigbolt, KT; Hu, B; Hlavacek, WS; Blagoev, B
Testing null

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