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Merck
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Supelco

Chromolith® WP 300 Epoxy (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

동의어(들):

Chromolith® WP 300 Epoxy 25-4.6 HPLC column

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About This Item

UNSPSC 코드:
41115700
NACRES:
SB.52

product name

Chromolith® WP 300 Epoxy, L × I.D. 25 mm x 4.6 mm HPLC column

material

PEEK column

Quality Level

제품 라인

Chromolith®
Chromolith®

특징

endcapped: no
endcapped: no

포장

1 ea of

라벨링 범위

5-6% Carbon loading

파라미터

2 - 60 °C temp. range
2-60 °C temp. range
200 bar pressure
200 bar pressure

기술

HPLC: suitable
HPLC: suitable
LC/MS: suitable

컬럼 길이 × I.D.

2.5 cm × 4.6 mm

표면적

120 m2/g

기질

monolithic silica
monolithic silica

기질 활성군

Epoxy bonding phase
Epoxy bonding phase

공극 크기

2 μm macroporosity
2 μm macroporosity
30 nm mesoporosity
30 nm mesoporosity

operating pH range

1.5-7.5
1.5-7.5

저장 온도

no temp limit

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일반 설명

Chromolith® WP 300 Epoxy columns are specially designed for the user-specific immobilization of ligands and their later application in HPLC. The unique bimodal pore structure of silica monoliths allows efficient coupling independent of molecule size. The wider mesopores also enable the use of proteins and antibodies as both ligand immobilized on the column, and later analyte separated by an immobilized column.
Potential applications: attach trypsin to obtain HPLC column-protein digestion reactor; attach protein and measure other protein interaction with the attached one; attach any chiral selector to obtain a chiral column; attach any affinity ligand to obtain custom made affinity column, etc.

Chromolith® WP 300 Epoxy 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Easy to perform immobilization of ligand opens up limitless customization options
—Monolithic format allows for high flow rates at low backpressure, enabling high throughput

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Epoxy is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Create Your Own Column Chemistry - Chromolith® WP 300 Epoxy 2 mm I.D. HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

법적 정보

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

시험 성적서(COA)

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문서

Silica monoliths with larger mesopores are a powerful tool for the fast separation of biomolecules including mAbs. The immobilization of protein A on epoxy-modified widepore (WP) silica rods enabled highly reproducible and fast, one minute, separations of mAbs. The Chromolith® WP 300 is also available with a protein A phase.

관련 콘텐츠

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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