14-912-M

TGFBR-1 Protein, active, 10 µg

Active, N-terminal GST-tagged, recombinant, human TGFBR1 amino acids 200–end containing a T204D mutation, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose. For use in Kinase Assays.

• 동의어(들): TGF-beta receptor type-1, TGFR-1, Transforming growth factor-beta receptor type I, TGF-beta receptor type I, TbetaR-I, TGF-beta type I receptor, Serine/threonine-protein kinase receptor R4, SKR4, Activin receptor-like kinase 5, ALK-5
• UNSPSC 코드: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in baculovirus infected Sf21 cells
• 분자량: M_w 62 kDa
• 제조업체/상표: Upstate^®
• 기술: activity assay: suitable (kinase)
• NCBI 수납 번호: NM_004612
• UniProt 수납 번호: P36897; Q64729 (mouse)
• 유전자 정보: human ... TGFBR1(7046)

설명

일반 설명

N-terminal GST-tagged, recombinant, human TGFBR1 amino acids 200–end containing a T204D mutation, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.

Product Source: Expressed by baculovirus in Sf21 insect cells.

Transforming growth factor, beta receptor type I (TGF-beta receptor I) forms a heteromeric complex with TGF-beta receptor type II when bound to TGF-beta. This complex transduces the TGF-beta signal from the cell surface to the cytoplasm. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. TGF-beta receptor I is found in all tissues, but is most abundant in placenta and least abundant in brain and heart tissue. TGF-beta receptor I is phosphorylated at basal levels in the absence of ligand binding and is multiply phosphorylated, mainly in the GS region. Defects in TGF-beta receptor I are the cause of Loeys-Dietz syndrome type 1A (LDS1A), also known as Furlong syndrome or Loeys-Dietz aortic aneurysm syndrome (LDAS).

애플리케이션

Research Category
Inflammation & Immunology

생화학적/생리학적 작용

Protein Target: TGFBR1

Target Sub-Family: TKL

포장

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-912M when ordering the 250 µg size.

품질

Routinely evaluated by phosphorylation of casein.

물리적 형태

50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Glutathione agarose affinity chromatography

저장 및 안정성

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

기타 정보

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.