09-843

Anti-SIRT2 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): SIR2-like protein 2, silencing information regulator 2-like, silent information regulator 2, sir2-related protein type 2, sirtuin (silent mating type information regulation 2, homolog) 2 (S. cerevisiae) ; ; sirtuin (silent mating type information r
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, mouse
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: NP_036369
• UniProt 수납 번호: Q8IXJ6
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SIRT2(22933)

설명

일반 설명

Sirtuins are hypothesized to play a key role in an organism′s response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Mammals have seven sirtuins; SIRT1 and SIRT2 occupy the cytoplasm. SIRT2 is a NAD-dependent deacytylase. It has been shown to control gene silencing, cell cycle, and DNA damage repair. In additon, SIRT2 acts as a tumor suppressor in human gliomas which suggests that the ectopic expression of SIRT2 upsets the microtubule network.

특이성

This antibody recognizes the mature protein of SIRT2.

면역원

Epitope: Mature protein

KLH-conjugated linear peptide corresponding to the mature protein of human SIRT2.

애플리케이션

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

Use Anti-SIRT2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect SIRT2 also known as SIR2-like protein 2, silent information regulator 2, sirtuin 2.

Western Blot (SNAP ID) Analysis: 0.25 µg/mL from a representative lot detected SIRT2 on 10 µg of human brain tissue lysate.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated SIRT2 in human brain lysate.

품질

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 0.125 µg/mL of this antibody detected SIRT2 on 10 µg of human brain tissue lysate.

표적 설명

Observed molecular weight is ~39 kDa Multiple isoforms around 40 kDa may be observed.

결합

Replaces: 04-1124

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Human brain tissue lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable