07-722

Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody

Upstate^®, from rabbit

• 동의어(들): Kinase insert domain receptor, Protein-tyrosine kinase receptor Flk-1, kinase insert domain receptor (a type III receptor tyrosine kinase), vascular endothelial growth factor receptor 2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: zebrafish, human
• 종 반응성(상동성에 의해 예측): primate (based on 100% sequence homology), horse (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology), Danio (based on 100% sequence homology)
• 제조업체/상표: Upstate^®
• 기술: western blot: suitable
• NCBI 수납 번호: NM_002253.2
• UniProt 수납 번호: P35968
• 배송 상태: dry ice
• 타겟 번역 후 변형: phosphorylation (pTyr1054)
• 유전자 정보: human ... KDR(3791)

설명

일반 설명

The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 (also known as Flk-2) is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines.

특이성

Recognizes VEGFR2/Flk-1/KDR when phosphorylated on Tyrosine 1054 (human).

면역원

Synthetic peptide encompassing

애플리케이션

Anti-phospho-VEGFR2/Flk-1/KDR (Tyr1054) Antibody is an antibody against phospho-VEGFR2/Flk-1/KDR (Tyr1054) for use in WB.

Research Category
Signaling

품질

Western Blot Analysis:
This lot detected VEGFR2 phosphorylated on Tyr1054 at a 1:1,000 dilution in PAE cells transfected with CSF/KDR and treated with 40ng/mL CSF-1 for 15 min. Cell lysate resolved via SDS-PAGE and transferred to PVDF (Immobilon-P).

표적 설명

152 kDa

물리적 형태

Affinity purified rabbit polyclonal IgG in PBS with 0.05% NaN3 and 50% glycerol.

Double affinity purified

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
CSF-1 treated PAE cell lysate, transfected with CSF/KDR.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2