07-716-I

Anti-KDR/Flk-1/VEGFR2 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, FLK-1, Kinase NYK, Protein-tyrosine kinase receptor flk-1, CD309
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, human
• 기술: western blot: suitable
• UniProt 수납 번호: P35918
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... KDR(3791) ; mouse ... Kdr(16542)

설명

일반 설명

The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.

면역원

Epitope: C-terminus

GST-tagged recombinant protein corresponding to the C-terminus of mouse KDR/Flk-1/VEGFR2.

애플리케이션

Detect KDR/Flk-1/VEGFR2 using this rabbit polyclonal antibody, Anti-KDR/Flk-1/VEGFR2 Antibody validated for use in western blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Western Blotting Analysis: A 1:500 dilution from a representative lot detected KDR/Flk-1/VEGFR2 in 10 µg of PAE cells transfected with CSF/VEGFR2 chimera.

품질

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected KDR/Flk-1/VEGFR2 in 10 µg of HUVEC cell lysate.

표적 설명

~230 kDa observed

결합

Replaces: 07-716

물리적 형태

Affinity purified

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable