07-2273

Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)

from rabbit, purified by affinity chromatography

• 동의어(들): DCHT, Serine/threonine-protein kinase 39
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, human
• 종 반응성(상동성에 의해 예측): canine (based on 100% sequence homology), rat (based on 100% sequence homology)
• 기술: western blot: suitable
• NCBI 수납 번호: NP_037365
• UniProt 수납 번호: Q9UEW8
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer373)
• 유전자 정보: human ... STK39(27347)

설명

일반 설명

As members of the (sterile 20)-like (STE20) kinase family, the oxidative-stress-responsive kinase 1 (OSR1) and the SPS1-related proline/alanine-rich kinase (SPAK) serve as key enzymes in a signaling cascade, which mediate the activity of Na+/K+/2Cl- cotransporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which are 79% identical to each other at the sequence level, and are also highly conserved in orthologues of these enzymes in Caenorhabditis elegans, Drosophila, and Xenopus. The CCT domain reflects no similarity to sequences found in other proteins. This domain is known to recognize and interact with a unique peptide (Arg-Phe-Xaa-Val) motif, which is present in OSRT1- and SPAK-activating kinases (with-no-lysine-kinase 1 (WNK1 and WNK4) and also in STE20 kinase family substrates (NKCC1 and NKCC2). It is reported that phosphorylation of a Ser/Thr residue preceding the (Arg-Phe-Xaa-Val) motif results in steric clash, and subsequent dissociation from the CCT domain. Furthermore, patients suffering from Gordon syndrome carry mutated WNK1 and WNK4 genes.

특이성

This antibody recognizes SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.

면역원

Epitope: SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.

KLH-conjugated linear peptide corresponding to human SPAK and OSR1 phosphorylated at Ser375 and Ser325, respectively.

애플리케이션

Research Category
Signaling

Inflammation & Immunology

Research Sub Category
Kinases & Phosphatases

Oxidative Stress

This phospho-SPAK antibody is validated for use in WB for the detection of the phospho-SPAK protein.

품질

Evaluated by Western Blot in hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.

Western Blot Anlaysis: 0.5 µg/mL of this antibody detected SPAK / OSR1 in 10 µg hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.

표적 설명

~60 kDa observed

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Hypotonic-stimulated HEK293 cells untreated and lambda phosphatase-treated.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable