07-2051

Anti-PEMT Antibody (CT)

from rabbit

• 동의어(들): phosphatidylethanolamine N-methyltransferase
• UNSPSC 코드: 12352203
• eCl@ss: 32160702

속성

• 생물학적 소스: rabbit
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human
• 기술: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_009100.2
• UniProt 수납 번호: Q9UBM1
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PEMT(10400)

설명

일반 설명

PEMT (phosphatidylethanolamine N-methyltransferase) is an an important enzyme for the synthesis of membrane phospholipids. PEMT converts phosphatidylethanolamine to phosphatidyl-choline by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Inhibition of hepatic PEMT activity has been associated with Alzheimer’s disease and schizophrenia.

특이성

Other homologies: Mouse (86% sequence homology) and Rat (73% sequence homology).

This antibody recognizes the C-terminal region of human PEMT in Jurkat cell lysates. The antibody easily detected PEMT in Jurkat and L6 lysates by Western blotting, but expression was weak or not detected in A431, HeLa, HEK293, PC12, or NIH/3T3 cell lysates using very dilute antibody (0.25 μg/mL, data not shown). Positive signals in immunocytochemistry using higher concentrations of antibody (2.5 μg/mL) detected PEMT in these cell lines, suggesting differential expression levels of this protein in various cell lines.

면역원

Epitope: C-terminus

The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PEMT.

애플리케이션

ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA

Immunocytochemistry:
HeLa, A431, and NIH/3T3 (previous page) cells incubated with anti-PEMT (C-terminus) stained positively on nuclei and endoplasmic reticulum (ER).

Research Category
Epigenetics & Nuclear Function

Neuroscience

Research Sub Category
Chromatin Biology

This PEMT antibody is validated for use in WB, ICC & ELISA for the detection of the PEMT protein.

품질

Western Blot Analysis:
1:1,000 dilution of this antibody detected PEMT on 10 µg of L6 lysate (below) and Jurkat lysate (not shown).

표적 설명

~22 kDa

물리적 형태

Format: Purified

Protein A chromatography

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

저장 및 안정성

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

분석 메모

Control
L6 and Jurkat lysates (Western blotting), HeLa, A431 cells (Immunocytochemistry)

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable