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Merck
모든 사진(1)

주요 문서

07-1585

Sigma-Aldrich

Anti-HIF-1-alpha Antibody, hydroxyproline (Pro402)

from rabbit, purified by affinity chromatography

동의어(들):

Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

human

종 반응성(상동성에 의해 예측)

rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)

포장

antibody small pack of 25 μg

기술

western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

ambient

타겟 번역 후 변형

unmodified

유전자 정보

human ... HIF1A(3091)

일반 설명

Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with HIF-1β to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.

특이성

This antibody recognizes HIF-1-alpha at the ODD region.

면역원

Epitope: ODD region
KLH-conjugated linear peptide corresponding to the ODD region of human HIF-1-alpha.

애플리케이션

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-HIF-1-alpha Antibody, hydroxyproline (Pro402) (Rabbit Polyclonal Antibody) validated in WB to detect HIF-1-alpha also known as Hypoxia-inducible factor 1-alpha, HIF1-alpha.

품질

Evaluated by Western Blot in FeCl2 treated HIF-1-alpha recombinant proteins.

Western Blot Analysis: 1 µg/mL of this antibody detected HIF-1-alpha on 10 µg of FeCl2 treated HIF-1-alpha recombinant proteins.

표적 설명

~120 kDa observed using Gal4-tagged recombinant protein. 92 kDa calculated for native protein.

결합

Replaces: 04-1006

물리적 형태

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
FeCl2 treated HIF-1-alpha recombinant proteins

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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문서 라이브러리 방문

Proline-hydroxylated hypoxia-inducible factor 1? (HIF-1?) upregulation in human tumours.
Snell, CE; Turley, H; McIntyre, A; Li, D; Masiero, M; Schofield, CJ; Gatter, KC; Harris et al.
Testing null
Michael I Koukourakis et al.
Scientific reports, 6, 30986-30986 (2016-08-11)
The mechanism of Amifostine (WR-2721) mediated radioprotection is poorly understood. The effects of amifostine on human basal metabolism, mouse liver metabolism and on normal and tumor hepatic cells were studied. Indirect calorimetric canopy tests showed significant reductions in oxygen consumption
Linda O'Flaherty et al.
Human molecular genetics, 19(19), 3844-3851 (2010-07-28)
Mutations in the gene encoding the Krebs cycle enzyme fumarate hydratase (FH) predispose to hereditary leiomyomatosis and renal cell cancer in affected individuals. FH-associated neoplasia is characterized by defective mitochondrial function and by upregulation of transcriptional pathways mediated by hypoxia-inducible
Ya-Min Tian et al.
The Journal of biological chemistry, 286(15), 13041-13051 (2011-02-22)
Hypoxia inducible factor (HIF) is regulated by dual pathways involving oxygen-dependent prolyl and asparaginyl hydroxylation of its α-subunits. Prolyl hydroxylation at two sites within a central degradation domain promotes association of HIF-α with the von Hippel-Lindau ubiquitin E3 ligase and
A Lakoma et al.
Cell death discovery, 1 (2015-01-01)
Neuroblastoma is an aggressive pediatric malignancy which is >98% p53 wild-type at diagnosis. As a primary repressor of p53 activity and part of a p53-activated negative feedback loop, targeting of mouse double minute 2 homolog (MDM2) is an attractive therapeutic

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