추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, human, rat
기술
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... CDC42(998)
일반 설명
cdc42 is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal Actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of cdc42 stimulates filopodia formation, whereas Rac results in the formation of lamellipodia, and Rho stimulates formation of actin stress fibers. Effectors of the cdc42/Rac pathway include NADPH Oxidase (Phox) and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of cdc42.
특이성
Recognizes Cdc42, does not cross react with Rac1. Cdc42 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Cdc42 induces the formation of filopodia. Cdc42 can activate Rac (consequently, filopodia are intimately associated with lamellipodia.
면역원
Synthetic peptide corresponding to amino acids 130-141 of human Cdc42
(AKNKQKPITPET-C).
(AKNKQKPITPET-C).
애플리케이션
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
This Anti-Cdc42 Antibody is validated for use in WB for the detection of Cdc42.
Western Blot Analysis: Rat brain with previous lots of rabbit anti-Cdc42 (cat. # 07-1466) and rabbit anti-Rac1 (cat. # 07-14664).
The molecular weights of Rac1 and Cdc42 are ~21 kDa.
Optimal working dilutions must be determined by end user.
The molecular weights of Rac1 and Cdc42 are ~21 kDa.
Optimal working dilutions must be determined by end user.
품질
Routinely evaluated by Western Blot on rat brain lysates.
Western Blot Analysis: 1:500-1:2000 (0.5-2μg/mL) dilution of rat brain lysate with a previous lot of rabbit anti-Cdc42.
Western Blot Analysis: 1:500-1:2000 (0.5-2μg/mL) dilution of rat brain lysate with a previous lot of rabbit anti-Cdc42.
표적 설명
21 kDa
결합
Replaces: AB4201
물리적 형태
Affinity Purified
Format: Purified
Purified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative.
저장 및 안정성
Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
분석 메모
Control
Rat Brain Lysate
Rat Brain Lysate
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Edward O Kwarteng et al.
Journal of leukocyte biology, 103(3), 381-393 (2018-01-19)
The switch from fetal to adult hematopoietic stem/progenitor cells (HSPCs) is associated with profound changes in several genetic programs. Although HSPC ageing corresponds to alterations in Wnt signaling, relatively little is known about the relative roles of different Wnt signaling
Amanda Amoah et al.
Haematologica (2021-01-15)
In this study, we characterize age-related phenotypes of human hematopoietic stem cells (HSCs). We report increased frequencies of HSC, HPC and lineage negative cells in the elderly but a decreased frequency of multi-lymphoid progenitors. Aged human HSCs further exhibited a
Tione Buranda et al.
Analytical biochemistry, 442(2), 149-157 (2013-08-10)
We describe a rapid assay for measuring the cellular activity of small guanine triphosphatases (GTPases) in response to a specific stimulus. Effector-functionalized beads are used to quantify in parallel multiple GTP-bound GTPases in the same cell lysate by flow cytometry.
Yang Liu et al.
Developmental biology, 383(2), 239-252 (2013-09-24)
Neural crest cells (NCCs) are physically responsible for craniofacial skeleton formation, pharyngeal arch artery remodeling and cardiac outflow tract septation during vertebrate development. Cdc42 (cell division cycle 42) is a Rho family small GTP-binding protein that works as a molecular
p16 Stimulates CDC42-dependent migration of hepatocellular carcinoma cells.
Chen, YW; Chu, HC; Ze-Shiang Lin, ; Shiah, WJ; Chou, CP; Klimstra, DS; Lewis, BC
Testing null
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